MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence

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Pinheiro , J , Lisboa , J , Pombinho , R , Carvalho , F , Carreaux , A , Brito , C , Pöntinen , A , Korkeala , H , dos Santos , N M S , Morais-Cabral , J H , Sousa , S & Cabanes , D 2018 , ' MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence ' , Nucleic Acids Research , vol. 46 , no. 18 , pp. 9338-9352 . https://doi.org/10.1093/nar/gky624

Title: MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence
Author: Pinheiro, Jorge; Lisboa, Johnny; Pombinho, Rita; Carvalho, Filipe; Carreaux, Alexis; Brito, Cláudia; Pöntinen, Anna; Korkeala, Hannu; dos Santos, Nuno M. S.; Morais-Cabral, João H.; Sousa, Sandra; Cabanes, Didier
Contributor: University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Departments of Faculty of Veterinary Medicine
Date: 2018-10-12
Language: eng
Number of pages: 15
Belongs to series: Nucleic Acids Research
ISSN: 0305-1048
URI: http://hdl.handle.net/10138/272997
Abstract: The foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible ani- mals and humans. To survive and proliferate within hosts, this facultative intracellular pathogen tightly coordinates the expression of a complex regulatory network that controls the expression of virulence fac- tors. Here, we identified and characterized MouR, a novel virulence regulator of Lm. Through RNA-seq transcriptomic analysis, we determined the MouR regulon and demonstrated how MouR positively con- trols the expression of the Agr quorum sensing sys- tem (agrBDCA) of Lm. The MouR three-dimensional structure revealed a dimeric DNA-binding transcrip- tion factor belonging to the VanR class of the GntR superfamily of regulatory proteins. We also showed that by directly binding to the agr promoter region, MouR ultimately modulates chitinase activity and biofilm formation. Importantly, we demonstrated by in vitro cell invasion assays and in vivo mice infec- tions the role of MouR in Lm virulence.
Subject: BINDING
BIOFILM
EGD-E
GENE
GNTR FAMILY
IDENTIFICATION
RESPONSE-REGULATOR
RNA
STRESS TOLERANCE
TRANSCRIPTION REGULATORS
1182 Biochemistry, cell and molecular biology
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