Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG

Show full item record



Permalink

http://hdl.handle.net/10138/274087

Citation

Koistinen , H , Koel , M , Peters , M , Rinken , A , Lundin , K , Tuuri , T , Tapanainen , J S , Alfthan , H , Salumets , A , Stenman , U-H & Lavogina , D 2019 , ' Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG ' , Molecular and Cellular Endocrinology , vol. 479 , pp. 103-109 . https://doi.org/10.1016/j.mce.2018.09.006

Title: Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG
Author: Koistinen, Hannu; Koel, Mariann; Peters, Maire; Rinken, Ago; Lundin, Karolina; Tuuri, Timo; Tapanainen, Juha S.; Alfthan, Henrik; Salumets, Andres; Stenman, Ulf-Håkan; Lavogina, Darja
Contributor organization: Department of Clinical Chemistry and Hematology
Medicum
University of Helsinki
Department of Obstetrics and Gynecology
Clinicum
Timo Pyry Juhani Otonkoski / Principal Investigator
Reproductive Disease Modeling
HUS Gynecology and Obstetrics
HUS Abdominal Center
Date: 2019-01-05
Language: eng
Number of pages: 7
Belongs to series: Molecular and Cellular Endocrinology
ISSN: 0303-7207
DOI: https://doi.org/10.1016/j.mce.2018.09.006
URI: http://hdl.handle.net/10138/274087
Abstract: While human chorionic gonadotropin (hCG) appears to have an essential role in early pregnancy, it is controversial whether the hyperglycosylated form of hCG (hCG-h), which is the major hCG isoform during the first 4-5 weeks of pregnancy, is able to activate LH/hCG receptor (LHCGR). To address this, we utilized different extensively characterized hCG and hCG beta reference reagents, cell culture- and urine-derived hCG-h preparations, and an in vitro reporter system for LHCGR activation. The WHO hCG reference reagent (99/688) was found to activate LHCGR with an EC50-value of 3.3 +/- 0.6 pmol/L (n = 9). All three studied hCG-h preparations were also able to activate LHCGR, but with a lower potency (EC50-values between 7.1 +/- 0.5 and 14 +/- 3 pmol/L, n = 5-11, for all P <0.05 as compared to the hCG reference). The activities of commercial urinary hCG (Pregnyl) and recombinant hCG (Ovitrelle) preparations were intermediate between those of the hCG reference and the hCG-h. These results strongly suggest that the hCG-h is functionally similar to hCG, although it has lower potency for LHCGR activation. Whether this explains the reduced proportion of hCG-h to hCG reported in patients developing early onset pre-eclampsia or those having early pregnancy loss remains to be determined.
Subject: hCG
Hyperglycosylated hCG
LHCGR
LH/hCG receptor
Reporter system
HUMAN CHORIONIC-GONADOTROPIN
FREE BETA-SUBUNIT
HUMAN CHORIOGONADOTROPIN
GLYCOPROTEIN HORMONES
LUTEINIZING-HORMONE
BIOLOGICAL-ACTIVITY
1ST TRIMESTER
PREGNANCY
ISOFORMS
TROPHOBLAST
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S0303720718302764_main.pdf 1.130Mb PDF View/Open
Hyperglycosylat ... ower_activity_than_hCG.pdf 2.156Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record