Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG

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http://hdl.handle.net/10138/274087

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Koistinen , H , Koel , M , Peters , M , Rinken , A , Lundin , K , Tuuri , T , Tapanainen , J S , Alfthan , H , Salumets , A , Stenman , U-H & Lavogina , D 2019 , ' Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG ' , Molecular and Cellular Endocrinology , vol. 479 , pp. 103-109 . https://doi.org/10.1016/j.mce.2018.09.006

Title: Hyperglycosylated hCG activates LH/hCG-receptor with lower activity than hCG
Author: Koistinen, Hannu; Koel, Mariann; Peters, Maire; Rinken, Ago; Lundin, Karolina; Tuuri, Timo; Tapanainen, Juha S.; Alfthan, Henrik; Salumets, Andres; Stenman, Ulf-Håkan; Lavogina, Darja
Contributor: University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, Department of Obstetrics and Gynecology
University of Helsinki, Timo Pyry Juhani Otonkoski / Principal Investigator
University of Helsinki, Reproductive Disease Modeling
University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, Department of Obstetrics and Gynecology
University of Helsinki, Department of Clinical Chemistry and Hematology
Date: 2019-01-05
Language: eng
Number of pages: 7
Belongs to series: Molecular and Cellular Endocrinology
ISSN: 0303-7207
URI: http://hdl.handle.net/10138/274087
Abstract: While human chorionic gonadotropin (hCG) appears to have an essential role in early pregnancy, it is controversial whether the hyperglycosylated form of hCG (hCG-h), which is the major hCG isoform during the first 4-5 weeks of pregnancy, is able to activate LH/hCG receptor (LHCGR). To address this, we utilized different extensively characterized hCG and hCG beta reference reagents, cell culture- and urine-derived hCG-h preparations, and an in vitro reporter system for LHCGR activation. The WHO hCG reference reagent (99/688) was found to activate LHCGR with an EC50-value of 3.3 +/- 0.6 pmol/L (n = 9). All three studied hCG-h preparations were also able to activate LHCGR, but with a lower potency (EC50-values between 7.1 +/- 0.5 and 14 +/- 3 pmol/L, n = 5-11, for all P <0.05 as compared to the hCG reference). The activities of commercial urinary hCG (Pregnyl) and recombinant hCG (Ovitrelle) preparations were intermediate between those of the hCG reference and the hCG-h. These results strongly suggest that the hCG-h is functionally similar to hCG, although it has lower potency for LHCGR activation. Whether this explains the reduced proportion of hCG-h to hCG reported in patients developing early onset pre-eclampsia or those having early pregnancy loss remains to be determined.
Subject: hCG
Hyperglycosylated hCG
LHCGR
LH/hCG receptor
Reporter system
HUMAN CHORIONIC-GONADOTROPIN
FREE BETA-SUBUNIT
HUMAN CHORIOGONADOTROPIN
GLYCOPROTEIN HORMONES
LUTEINIZING-HORMONE
BIOLOGICAL-ACTIVITY
1ST TRIMESTER
PREGNANCY
ISOFORMS
TROPHOBLAST
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
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