Transient modification of lin28b expression - Permanent effects on zebrafish growth

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http://hdl.handle.net/10138/274089

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Leinonen , J T , Chen , Y-C , Tukiainen , T , Panula , P & Widen , E 2019 , ' Transient modification of lin28b expression - Permanent effects on zebrafish growth ' , Molecular and Cellular Endocrinology , vol. 479 , pp. 61-70 . https://doi.org/10.1016/j.mce.2018.09.001

Title: Transient modification of lin28b expression - Permanent effects on zebrafish growth
Author: Leinonen, Jaakko T.; Chen, Yu-Chia; Tukiainen, Taru; Panula, Pertti; Widen, Elisabeth
Contributor organization: Institute for Molecular Medicine Finland
University of Helsinki
Department of Anatomy
Neuroscience Center
Medicum
Helsinki In Vivo Animal Imaging Platform (HAIP)
Pertti Panula / Principal Investigator
Centre of Excellence in Complex Disease Genetics
Elisabeth Ingrid Maria Widen / Principal Investigator
Technology Centre
Genomic Discoveries and Clinical Translation
Date: 2019-01-05
Language: eng
Number of pages: 10
Belongs to series: Molecular and Cellular Endocrinology
ISSN: 0303-7207
DOI: https://doi.org/10.1016/j.mce.2018.09.001
URI: http://hdl.handle.net/10138/274089
Abstract: Recent genome-wide association studies and mouse models have identified LIN28B as a gene affecting several pubertal timing-related traits and vertebrate growth. However, the exact biological mechanisms underlying the associations remain unknown. We have explored the mechanisms linking LIN28B with growth regulation by combining human gene expression data with functional models. Specifically, we show that 1) pubertal timing-associated genetic variation correlates with LIN28B expression in the pituitary and hypothalamus, 2) downregulating lin28b in zebrafish embryos associates with aberrant development of kiss2-neurons, and 3) increasing lin28b expression transiently by synthetic mRNA injections during embryogenesis results in sustained enhancement of zebrafish growth. Unexpectedly, the mRNA injections resulted in advanced sexual maturation of female fish, suggesting that lin28b may influence pubertal timing through multiple developmental mechanisms. Overall, these results provide novel insight into LIN28B function in vertebrate growth regulation, emphasizing the importance of the gene and related genetic pathways for embryonic and juvenile development.
Subject: Lin28b
Zebrafish
Development
Hypothalamus
Kiss2
HYPOGONADOTROPIC HYPOGONADISM
EMBRYONIC-DEVELOPMENT
LET-7 MICRORNA
LOCI
HEIGHT
AGE
PHENOTYPES
PROTEINS
NEURONS
SYSTEM
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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