Immunoproteomics and surfaceomics of the adult tapeworm Hymenolepis diminuta

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Mlocicki , D , Sulima , A , Bień , J , Näreaho , A , Zawistowska-Deniziak , A , Basałaj , K , Sałamatin , R , Conn , D B & Savijoki , K 2018 , ' Immunoproteomics and surfaceomics of the adult tapeworm Hymenolepis diminuta ' , Frontiers in Immunology , vol. 9 , 2487 . https://doi.org/10.3389/fimmu.2018.02487

Title: Immunoproteomics and surfaceomics of the adult tapeworm Hymenolepis diminuta
Author: Mlocicki, Daniel; Sulima, Anna; Bień, Justyna; Näreaho, Anu; Zawistowska-Deniziak, Anna; Basałaj, Katarzyna; Sałamatin, Rusłan; Conn, David Bruce; Savijoki, Kirsi
Contributor organization: Veterinary Pathology and Parasitology
Veterinary Biosciences
Departments of Faculty of Veterinary Medicine
Faculty of Veterinary Medicine
Division of Pharmaceutical Biosciences
Pharmaceutical Design and Discovery group
Faculty of Pharmacy
Date: 2018-11-12
Language: eng
Number of pages: 14
Belongs to series: Frontiers in Immunology
ISSN: 1664-3224
DOI: https://doi.org/10.3389/fimmu.2018.02487
URI: http://hdl.handle.net/10138/274997
Abstract: In cestodiasis, mechanical and molecular contact between the parasite and the host activates the immune response of the host and may result in inflammatory processes, leading to ulceration and intestinal dysfunctions. The aim of the present study was to identify antigenic proteins of the adult cestode Hymenolepis diminuta by subjecting the total protein extracts from adult tapeworms to 2DE immunoblotting (two-dimensional electrophoresis combined with immunoblotting) using sera collected from experimentally infected rats. A total of 36 protein spots cross-reacting with the rat sera were identified using LC-MS/MS. As a result, 68 proteins, including certain structural muscle proteins (actin, myosin, and paramyosin) and moonlighters (heat shock proteins, kinases, phosphatases, and glycolytic enzymes) were identified; most of these were predicted to possess binding and/or catalytic activity required in various metabolic and cellular processes, and reported here as potential antigens of the adult cestode for the first time. As several of these antigens can also be found at the cell surface, the surface-associated proteins were extracted and subjected to in-solution digestion for LC-MS/MS identification (surfaceomics). As a result, a total of 76 proteins were identified, from which 31 proteins, based on 2DE immunoblotting, were predicted to be immunogenic. These included structural proteins actin, myosin and tubulin as well as certain moonlighting proteins (heat-shock chaperones) while enzymes with diverse catalytic activities were found as the most dominating group of proteins. In conclusion, the present study shed new light into the complexity of the enteric cestodiasis by showing that the H. diminuta somatic proteins exposed to the host possess immunomodulatory functions, and that the immune response of the host could be stimulated by diverse mechanisms, involving also those triggering protein export via yet unknown pathways.
Subject: DEVELOPMENTAL-STAGES
ECHINOCOCCUS-GRANULOSUS
HELMINTH-PARASITES
Hymenolepis diminuta
IN-VITRO
LARGE-INTESTINES
MOONLIGHTING PROTEINS
PROTEOMIC ANALYSIS
RAT TAPEWORM
RECEPTOR GENES TLR2
SCHISTOSOMA-JAPONICUM
cestoda
host-parasite interactions
immunoblotting
mass spectrometry
proteomics
surface proteins
tapeworm
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
413 Veterinary science
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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