Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds

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http://hdl.handle.net/10138/276982

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Ilina , P , Ma , X , Sintim , H O & Tammela , P S M 2018 , ' Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds ' , Journal of Microbiological Methods , vol. 154 , pp. 40-45 . https://doi.org/10.1016/j.mimet.2018.10.005

Title: Miniaturized whole-cell bacterial bioreporter assay for identification of quorum sensing interfering compounds
Author: Ilina, Polina; Ma, Xiaochu; Sintim, Herman O.; Tammela, Päivi Sirpa Marjaana
Contributor: University of Helsinki, Bioactivity Screening Group
University of Helsinki, Faculty of Pharmacy
Date: 2018-11
Language: eng
Number of pages: 6
Belongs to series: Journal of Microbiological Methods
ISSN: 0167-7012
URI: http://hdl.handle.net/10138/276982
Abstract: The continuing emergence and spread of antibiotic-resistant bacteria is worrisome and new strategies to curb bacterial infections are being sought. The interference of bacterial quorum sensing (QS) signaling has been suggested as a prospective antivirulence strategy. The AI-2 QS system is present in multiple bacterial species and has been shown to be correlated with pathogenicity. To facilitate the discovery of novel compounds interfering with AI-2 QS, we established a high-throughput setup of whole-cell bioreporter assay, which can be performed in either 96- or 384-well format. Agonistic or antagonistic activities of the test compounds against Escherichia coli LsrB-type AI-2 QS system are monitored by measuring the level of beta-galactosidase expression. A control strain expressing beta-galactosidase in quorum sensing-independent manner is included into the assay for false-positive detection.
Subject: AI-2
ART
AUTOINDUCER-2
Assay miniaturization
Autoinducer-2
Bioreporter strain
High throughput screening
INHIBITORS
PERSPECTIVES
Quorum sensing inhibitors
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
317 Pharmacy
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