Dual-trap optical tweezers with real-time force clamp control

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dc.contributor University of Helsinki, Department of Physics (-2009) en
dc.contributor University of Helsinki, Institute of Biotechnology (-2009) en
dc.contributor University of Helsinki, Biosciences en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor.author Wallin, Anders
dc.contributor.author Ojala, Heikki Viljami
dc.contributor.author Ziedaite, Gabija
dc.contributor.author Haeggström, Edward
dc.date.accessioned 2011-09-22T12:07:05Z
dc.date.available 2011-09-22T12:07:05Z
dc.date.issued 2011-08-03
dc.identifier.citation Wallin , A , Ojala , H V , Ziedaite , G & Haeggström , E 2011 , ' Dual-trap optical tweezers with real-time force clamp control ' Review of Scientific Instruments , vol. 82 , no. 8 , pp. 083102 . DOI: 10.1063/1.3615309 en
dc.identifier.issn 0034-6748
dc.identifier.other PURE: 16740542
dc.identifier.other PURE UUID: 100b2ca9-f311-485f-b597-24f7372bffcd
dc.identifier.other WOS: 000294486600003
dc.identifier.other Scopus: 80052402204
dc.identifier.uri http://hdl.handle.net/10138/27789
dc.description.abstract Single molecule force clamp experiments are widely used to investigate how enzymes, molecular motors, and other molecular mechanisms work. We developed a dual-trap optical tweezers instrument with real-time (200 kHz update rate) force clamp control that can exert 0–100 pN forces on trapped beads. A model for force clamp experiments in the dumbbell-geometry is presented. We observe good agreement between predicted and observed power spectra of bead position and force fluctuations. The model can be used to predict and optimize the dynamics of real-time force clamp optical tweezers instruments. The results from a proof-of-principle experiment in which lambda exonuclease converts a double-stranded DNA tether, held at constant tension, into its single-stranded form, show that the developed instrument is suitable for experiments in single molecule biology. en
dc.format.extent 7
dc.language.iso eng
dc.relation.ispartof Review of Scientific Instruments
dc.relation.uri http://link.aip.org/link/doi/10.1063/1.3615309
dc.rights en
dc.subject 114 Physical sciences en
dc.title Dual-trap optical tweezers with real-time force clamp control en
dc.type Article
dc.description.version Peer reviewed
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1063/1.3615309
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/acceptedVersion
dc.type.uri info:eu-repo/semantics/acceptedVersion
dc.contributor.pbl

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