PD-L1(+) tumor-associated macrophages and PD-1(+) tumor-infiltrating lymphocytes predict survival in primary testicular lymphoma

Show full item record



Permalink

http://hdl.handle.net/10138/278905

Citation

Pollari , M , Brück , O , Pellinen , T , Vähämurto , P , Karjalainen-Lindsberg , M-L , Mannisto , S , Kallioniemi , O , Kellokumpu-Lehtinen , P-L , Mustjoki , S , Leivonen , S-K & Leppä , S 2018 , ' PD-L1(+) tumor-associated macrophages and PD-1(+) tumor-infiltrating lymphocytes predict survival in primary testicular lymphoma ' , Haematologica , vol. 103 , no. 11 , pp. 1908-1914 . https://doi.org/10.3324/haematol.2018.197194

Title: PD-L1(+) tumor-associated macrophages and PD-1(+) tumor-infiltrating lymphocytes predict survival in primary testicular lymphoma
Author: Pollari, Marjukka; Brück, Oscar; Pellinen, Teijo; Vähämurto, Pauli; Karjalainen-Lindsberg, Marja-Liisa; Mannisto, Susanna; Kallioniemi, Olli; Kellokumpu-Lehtinen, Pirkko-Liisa; Mustjoki, Satu; Leivonen, Suvi-Katri; Leppä, Sirpa
Other contributor: University of Helsinki, University of Helsinki
University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Department of Oncology
University of Helsinki, Medicum
University of Helsinki, Sirpa Marianne Leppä / Principal Investigator
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, Research Programs Unit
University of Helsinki, Department of Oncology














Date: 2018-10-31
Language: eng
Number of pages: 7
Belongs to series: Haematologica
ISSN: 0390-6078
DOI: https://doi.org/10.3324/haematol.2018.197194
URI: http://hdl.handle.net/10138/278905
Abstract: Primary testicular lymphoma is a rare and aggressive lymphoid malignancy, most often representing diffuse large B-cell lymphoma histologically. Tumor-associated macrophages and tumor-infiltrating lymphocytes have been associated with survival in diffuse large B-cell lymphoma, but their prognostic impact in primary testicular lymphoma is unknown. Here, we aimed to identify macrophages, their immunophenotypes and association with lymphocytes, and translate the findings into survival of patients with primary testicular lymphoma. We collected clinical data and tumor tissue from 74 primary testicular lymphoma patients, and used multiplex immunohistochemistry and digital image analysis to examine macrophage markers (CD68, CD163, and c-Maf), T-cell markers (CD3, CD4, and CD8), B-cell marker (CD20), and three checkpoint molecules (PD-L1, PD-L2, and PD-1). We demonstrate that a large proportion of macrophages (median 41%, range 0.08-99%) and lymphoma cells (median 34%, range 0.1-100%) express PD-L1. The quantity of PD-L1+CD68+ macrophages correlates positively with the amount of PD-1+ lymphocytes, and a high proportion of either PD-L1+CD68+ macrophages or PD-1+CD4+ and PD-1+CD8+ T-cells translates into favorable survival. In contrast, the number of PD-L1+ lymphoma cells or PD-L1- macrophages do not associate with outcome. In multivariate analyses with IPI, PD-L1+CD68+ macrophage and PD-1+ lymphocyte contents remain as independent prognostic factors for survival. In conclusion, high PD-L1+CD68+ macrophage and PD-1+ lymphocyte contents predict favorable survival in patients with primary testicular lymphoma. The findings implicate that the tumor microenvironment and PD-1. PD-L1 pathway have a significant role in regulating treatment outcome. They also bring new insights to the targeted therapy of primary testicular lymphoma.
Subject: B-CELL LYMPHOMA
CENTRAL-NERVOUS-SYSTEM
DEATH LIGAND 1
CONTRALATERAL TESTIS
EXPRESSION
BLOCKADE
IRRADIATION
FEATURES
3122 Cancers
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
haematol.2018.197194.full.pdf 1.328Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record