NMR structure of the C-terminal domain of TonB protein from Pseudomonas aeruginosa

Show full item record



Permalink

http://hdl.handle.net/10138/279454

Citation

Oeemig , J S , Ollila , O H S & Iwaï , H 2018 , ' NMR structure of the C-terminal domain of TonB protein from Pseudomonas aeruginosa ' , PeerJ , vol. 6 , e5412 . https://doi.org/10.7717/peerj.5412

Title: NMR structure of the C-terminal domain of TonB protein from Pseudomonas aeruginosa
Author: Oeemig, Jesper S.; Ollila, O.H. Samuli; Iwaï, Hideo
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2018-08-27
Language: eng
Number of pages: 19
Belongs to series: PeerJ
ISSN: 2167-8359
URI: http://hdl.handle.net/10138/279454
Abstract: The TonB protein plays an essential role in the energy transduction system to drive active transport across the outer membrane (OM) using the proton-motive force of the cytoplasmic membrane of Gram-negative bacteria. The C-terminal domain (CTD) of TonB protein is known to interact with the conserved TonB box motif of TonB-dependent OM transporters, which likely induces structural changes in the OM transporters. Several distinct conformations of differently dissected CTDs of Escherichia coli TonB have been previously reported. Here we determined the solution NMR structure of a 96-residue fragment of Pseudomonas aeruginosa TonB (PaTonB-96). The structure shows a monomeric structure with the flexible C-terminal region (residues 338-342), different from the NMR structure of E. coli TonB (EcTonB-137). The extended and flexible C-terminal residues are confirmed by N-15 relaxation analysis and molecular dynamics simulation. We created models for the PaTonB-96/TonB box interaction and propose that the internal fluctuations of PaTonB-96 makes it more accessible for the interactions with the TonB box and possibly plays a role in disrupting the plug domain of the TonB-dependent OM transporters.
Subject: TonB
NMR
NMR structure
N-15 relaxation
Molecular dynamics
BtuB
Pseudomonas aeruginosa
TonB-dependent energy transduction
Protein structure
Outer membrane transporter
PARTICLE MESH EWALD
OUTER-MEMBRANE
ESCHERICHIA-COLI
MOLECULAR-DYNAMICS
ENERGY TRANSDUCTION
BACKBONE DYNAMICS
CRYSTAL-STRUCTURE
COMPLEX
SPECTROSCOPY
TRANSPORTER
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
peerj_5412.pdf 5.927Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record