Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Show simple item record

dc.contributor.author Gomez, Miguel
dc.contributor.author Leung, Whinkie
dc.contributor.author Dantuluri, Swathi
dc.contributor.author Pillai, Alexander
dc.contributor.author Gani, Zyan
dc.contributor.author Hwang, Sungmin
dc.contributor.author McMillan, Lana J.
dc.contributor.author Kiljunen, Saija
dc.contributor.author Savilahti, Harri
dc.contributor.author Maupin-Furlow, Julie A.
dc.date.accessioned 2019-01-04T11:02:01Z
dc.date.available 2019-01-04T11:02:01Z
dc.date.issued 2018-11
dc.identifier.citation Gomez , M , Leung , W , Dantuluri , S , Pillai , A , Gani , Z , Hwang , S , McMillan , L J , Kiljunen , S , Savilahti , H & Maupin-Furlow , J A 2018 , ' Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii ' , Genes , vol. 9 , no. 11 , 562 . https://doi.org/10.3390/genes9110562
dc.identifier.other PURE: 120004811
dc.identifier.other PURE UUID: 10a9ce49-b729-48c1-9b99-3365ce010b60
dc.identifier.other Bibtex: urn:c2659929509402b03f76df624f892084
dc.identifier.other Scopus: 85057080446
dc.identifier.other ORCID: /0000-0003-0461-7270/work/52397912
dc.identifier.other WOS: 000451636700047
dc.identifier.uri http://hdl.handle.net/10138/286186
dc.description.abstract Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a Delta psmA1 (alpha 1) markerless deletion strain that produces only the alpha 2 and beta proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces alpha 1, alpha 2, and beta proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof Genes
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 20S PROTEASOMES
dc.subject CRYSTAL-STRUCTURE
dc.subject FREE-RADICALS
dc.subject HALOBACTERIUM
dc.subject INTEGRATION
dc.subject OXIDATIVE STRESS
dc.subject PREDICTION
dc.subject PROTEIN
dc.subject TRANSPORTERS
dc.subject VISUALIZATION
dc.subject archaea
dc.subject hypochlorite
dc.subject oxidative stress
dc.subject proteasome
dc.subject redox-active
dc.subject 3111 Biomedicine
dc.title Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Department of Bacteriology and Immunology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/genes9110562
dc.relation.issn 2073-4425
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
genes_09_00562_v2.pdf 3.386Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record