Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Show simple item record Gomez, Miguel Leung, Whinkie Dantuluri, Swathi Pillai, Alexander Gani, Zyan Hwang, Sungmin McMillan, Lana J. Kiljunen, Saija Savilahti, Harri Maupin-Furlow, Julie A. 2019-01-04T11:02:01Z 2019-01-04T11:02:01Z 2018-11
dc.identifier.citation Gomez , M , Leung , W , Dantuluri , S , Pillai , A , Gani , Z , Hwang , S , McMillan , L J , Kiljunen , S , Savilahti , H & Maupin-Furlow , J A 2018 , ' Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii ' , Genes , vol. 9 , no. 11 , 562 .
dc.identifier.other PURE: 120004811
dc.identifier.other PURE UUID: 10a9ce49-b729-48c1-9b99-3365ce010b60
dc.identifier.other Bibtex: urn:c2659929509402b03f76df624f892084
dc.identifier.other Scopus: 85057080446
dc.identifier.other ORCID: /0000-0003-0461-7270/work/52397912
dc.identifier.other WOS: 000451636700047
dc.description.abstract Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a Delta psmA1 (alpha 1) markerless deletion strain that produces only the alpha 2 and beta proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces alpha 1, alpha 2, and beta proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof Genes
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 20S PROTEASOMES
dc.subject FREE-RADICALS
dc.subject INTEGRATION
dc.subject PREDICTION
dc.subject PROTEIN
dc.subject archaea
dc.subject hypochlorite
dc.subject oxidative stress
dc.subject proteasome
dc.subject redox-active
dc.subject 3111 Biomedicine
dc.title Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Department of Bacteriology and Immunology
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2073-4425
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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