Validation of serological and molecular methods for diagnosis of zika virus infections

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dc.contributor.author Jääskeläinen, Anne J.
dc.contributor.author Korhonen, Essi M.
dc.contributor.author Huhtamo, Eili
dc.contributor.author Lappalainen, Maija
dc.contributor.author Vapalahti, Olli
dc.contributor.author Kallio-Kokko, Hannimari
dc.date.accessioned 2019-01-07T11:43:01Z
dc.date.available 2019-01-07T11:43:01Z
dc.date.issued 2019-01
dc.identifier.citation Jääskeläinen , A J , Korhonen , E M , Huhtamo , E , Lappalainen , M , Vapalahti , O & Kallio-Kokko , H 2019 , ' Validation of serological and molecular methods for diagnosis of zika virus infections ' , Journal of Virological Methods , vol. 263 , pp. 68-74 . https://doi.org/10.1016/j.jviromet.2018.10.011
dc.identifier.other PURE: 120795179
dc.identifier.other PURE UUID: ef2c0288-08ad-436e-9f73-fb739c475baa
dc.identifier.other WOS: 000452936300012
dc.identifier.other Scopus: 85055916989
dc.identifier.other ORCID: /0000-0003-2270-6824/work/52396061
dc.identifier.other ORCID: /0000-0001-5400-1250/work/52396839
dc.identifier.other ORCID: /0000-0001-9640-6547/work/101081136
dc.identifier.other ORCID: /0000-0003-4730-7715/work/110391072
dc.identifier.uri http://hdl.handle.net/10138/287679
dc.description.abstract The laboratory confirmation of Zika virus (ZIKV) infection, and the differential diagnosis from other flavivirus infections such as dengue virus (DENV), often requires the use of several diagnostic test types. Cross-reactions and secondary infections complicate the serological diagnosis and specific viral RNA detection assays are often needed for confirming the diagnosis. The aim of this study was to validate serological and molecular methods for diagnosing ZIKV infection. This included the evaluation of a ZIKV RT-qPCR assay for diagnostics that was previously set up for research use and to compare the ZIKV, DENV and TBEV EIA methods. External and in-house controls and pre-characterized sample panels were tested, and also automated and manual nucleic acid extraction methods were compared. A total of ten Finnish traveler patients were diagnosed with acute ZIKV infection during 2015-2017 including one suspected dual DENV and ZIKV infection. These samples along with panels of DENV and tick-bome encephalitis virus (TBEV) infections were used to test the cross-reactive properties of ZIKV, DENV and TBEV IgM assays. Additionally, the diagnosed acute ZIKV patient samples were tested using commercially available diagnostic DENV NS1 antigen assay and a ZIKV NS1 antigen assay intended for research use. The ZIKV RT-qPCR assay was demonstrated to be both specific and sensitive (one genome per reaction) and suitable for routine diagnostic use utilizing automated nucleic acid extraction. Of the tested IgM tests the NS1 antigen-based ZIKV IgM (Euroimmun) assay performed with least cross -reactivity with a specificity of 97.4%. The DENV IgM assay (Focus Diagnostics) had specificity of only 86.1%. The results are in line with previous studies and additionally highlight that also acute TBEV patients may give a false positive test result in DENV and ZIKV IgM assays. en
dc.format.extent 7
dc.language.iso eng
dc.relation.ispartof Journal of Virological Methods
dc.rights cc_by_nc_nd
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Zika
dc.subject IgM
dc.subject RT-qPCR
dc.subject Diagnosis
dc.subject Dengue
dc.subject TBEV
dc.subject TIME RT-PCR
dc.subject CLINICAL PRESENTATION
dc.subject WHOLE-BLOOD
dc.subject DENGUE
dc.subject VIREMIA
dc.subject SPECIFICITY
dc.subject COINFECTION
dc.subject RNA
dc.subject IGM
dc.subject 3111 Biomedicine
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 1183 Plant biology, microbiology, virology
dc.title Validation of serological and molecular methods for diagnosis of zika virus infections en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization HUSLAB
dc.contributor.organization Viral Zoonosis Research Unit
dc.contributor.organization Department of Virology
dc.contributor.organization University of Helsinki
dc.contributor.organization Clinicum
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Helsinki One Health (HOH)
dc.contributor.organization Veterinary Microbiology and Epidemiology
dc.contributor.organization Olli Pekka Vapalahti / Principal Investigator
dc.contributor.organization Faculty of Medicine
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.jviromet.2018.10.011
dc.relation.issn 0166-0934
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion
dc.type.version publishedVersion

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