Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles

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Jiu , Y , Kumari , R , Fenix , A M , Schaible , N , Liu , X , Varjosalo , M , Krishnan , R , Burnette , D T & Lappalainen , P 2019 , ' Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles ' , Current Biology , vol. 29 , no. 1 , pp. 81-92 . https://doi.org/10.1016/j.cub.2018.11.045

Title: Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles
Author: Jiu, Yaming; Kumari, Reena; Fenix, Aidan M.; Schaible, Niccole; Liu, Xiaonan; Varjosalo, Markku; Krishnan, Ramaswamy; Burnette, Dylan T.; Lappalainen, Pekka
Other contributor: University of Helsinki, Staff Services
University of Helsinki, Institute of Biotechnology
University of Helsinki, Doctoral Programme in Integrative Life Science
University of Helsinki, Institute of Biotechnology
University of Helsinki, Doctoral Programme in Integrative Life Science







Date: 2019-01
Language: eng
Number of pages: 12
Belongs to series: Current Biology
ISSN: 0960-9822
DOI: https://doi.org/10.1016/j.cub.2018.11.045
URI: http://hdl.handle.net/10138/288205
Abstract: Summary Cell adhesion, morphogenesis, mechanosensing, and muscle contraction rely on contractile actomyosin bundles, where the force is produced through sliding of bipolar myosin II filaments along actin filaments. The assembly of contractile actomyosin bundles involves registered alignment of myosin II filaments and their subsequent fusion into large stacks. However, mechanisms underlying the assembly of myosin II stacks and their physiological functions have remained elusive. Here, we identified myosin-18B, an unconventional myosin, as a stable component of contractile stress fibers. Myosin-18B co-localized with myosin II motor domains in stress fibers and was enriched at the ends of myosin II stacks. Importantly, myosin-18B deletion resulted in drastic defects in the concatenation and persistent association of myosin II filaments with each other and thus led to severely impaired assembly of myosin II stacks. Consequently, lack of myosin-18B resulted in defective maturation of actomyosin bundles from their precursors in osteosarcoma cells. Moreover, myosin-18B knockout cells displayed abnormal morphogenesis, migration, and ability to exert forces to the environment. These results reveal a critical role for myosin-18B in myosin II stack assembly and provide evidence that myosin II stacks are important for a variety of vital processes in cells.
Subject: myosin-18B
actin
stress fiber
actomyosin bundles
myosin II
cytoskeleton
mechanosensing
cell migration
1182 Biochemistry, cell and molecular biology
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