Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles

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Jiu , Y , Kumari , R , Fenix , A M , Schaible , N , Liu , X , Varjosalo , M , Krishnan , R , Burnette , D T & Lappalainen , P 2019 , ' Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles ' , Current Biology , vol. 29 , no. 1 , pp. 81-92 . https://doi.org/10.1016/j.cub.2018.11.045

Titel: Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles
Författare: Jiu, Yaming; Kumari, Reena; Fenix, Aidan M.; Schaible, Niccole; Liu, Xiaonan; Varjosalo, Markku; Krishnan, Ramaswamy; Burnette, Dylan T.; Lappalainen, Pekka
Upphovmannens organisation: Staff Services
Institute of Biotechnology
Doctoral Programme in Integrative Life Science
Doctoral Programme in Biomedicine
Doctoral Programme in Drug Research
University Management
Molecular Systems Biology
Pekka Lappalainen / Principal Investigator
Datum: 2019-01
Språk: eng
Sidantal: 12
Tillhör serie: Current Biology
ISSN: 0960-9822
DOI: https://doi.org/10.1016/j.cub.2018.11.045
Permanenta länken (URI): http://hdl.handle.net/10138/288205
Abstrakt: Summary Cell adhesion, morphogenesis, mechanosensing, and muscle contraction rely on contractile actomyosin bundles, where the force is produced through sliding of bipolar myosin II filaments along actin filaments. The assembly of contractile actomyosin bundles involves registered alignment of myosin II filaments and their subsequent fusion into large stacks. However, mechanisms underlying the assembly of myosin II stacks and their physiological functions have remained elusive. Here, we identified myosin-18B, an unconventional myosin, as a stable component of contractile stress fibers. Myosin-18B co-localized with myosin II motor domains in stress fibers and was enriched at the ends of myosin II stacks. Importantly, myosin-18B deletion resulted in drastic defects in the concatenation and persistent association of myosin II filaments with each other and thus led to severely impaired assembly of myosin II stacks. Consequently, lack of myosin-18B resulted in defective maturation of actomyosin bundles from their precursors in osteosarcoma cells. Moreover, myosin-18B knockout cells displayed abnormal morphogenesis, migration, and ability to exert forces to the environment. These results reveal a critical role for myosin-18B in myosin II stack assembly and provide evidence that myosin II stacks are important for a variety of vital processes in cells.
Subject: myosin-18B
actin
stress fiber
actomyosin bundles
myosin II
cytoskeleton
mechanosensing
cell migration
1182 Biochemistry, cell and molecular biology
Referentgranskad: Ja
Licens: cc_by_nc_nd
Användningsbegränsning: openAccess
Parallelpublicerad version: draft


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