Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation

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Biggs , L C , Mäkelä , O J M , Myllymäki , S-M , Das Roy , R , Närhi , K , Pispa , J , Mustonen , T & Mikkola , M L 2018 , ' Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation ' , eLife , vol. 7 , 36468 . https://doi.org/10.7554/eLife.36468

Title: Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation
Author: Biggs, Leah C.; Mäkelä, Otto J. M.; Myllymäki, Satu-Marja; Das Roy, Rishi; Närhi, Katja; Pispa, Johanna; Mustonen, Tuija; Mikkola, Marja L.
Contributor: University of Helsinki, Helsinki Institute of Life Science HiLIFE, Tenure Track
University of Helsinki, Doctoral Programme in Integrative Life Science
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Translational Cancer Biology (TCB) Research Programme
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Doctoral Programme in Integrative Life Science
Date: 2018-07-31
Language: eng
Number of pages: 33
Belongs to series: eLife
ISSN: 2050-084X
URI: http://hdl.handle.net/10138/288216
Abstract: Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling throughout life and bears hair inductive potential. Dermal condensate morphogenesis depends on epithelial Fibroblast Growth Factor 20 (Fgf20). Here, we combine mouse models with 3D and 4D microscopy to demonstrate that dermal condensates form de novo and via directional migration. We identify cell cycle exit and cell shape changes as early hallmarks of dermal condensate morphogenesis and find that Fgf20 primes these cellular behaviors and enhances cell motility and condensation. RNAseq profiling of immediate Fgf20 targets revealed induction of a subset of dermal condensate marker genes. Collectively, these data indicate that dermal condensation occurs via directed cell movement and that Fgf20 orchestrates the early cellular and molecular events.
Subject: PAPILLA CELLS
SONIC-HEDGEHOG
BETA-CATENIN
MESENCHYMAL CONDENSATION
FEATHER DEVELOPMENT
DYNAMIC EXPRESSION
PATTERN-FORMATION
GENE-EXPRESSION
RECEPTOR EDAR
NEURAL CREST
1182 Biochemistry, cell and molecular biology
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