Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)

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Eskelin , K J & Poranen , M M 2018 , ' Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) ' , Viruses-Basel , vol. 10 , no. 11 , 579 . https://doi.org/10.3390/v10110579

Title: Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)
Author: Eskelin, Katri Johanna; Poranen, Minna Marjetta
Other contributor: University of Helsinki, Doctoral Programme in Microbiology and Biotechnology
University of Helsinki, Molecular and Integrative Biosciences Research Programme




Date: 2018-11
Language: eng
Number of pages: 13
Belongs to series: Viruses-Basel
ISSN: 1999-4915
DOI: https://doi.org/10.3390/v10110579
URI: http://hdl.handle.net/10138/289800
Abstract: Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer. Controlled dissociation of virions provides important information on virion composition, interactions, and stoichiometry of virion components, as well as their possible role in virus life cycles. Dissociation of viruses can be achieved by using various chemicals, enzymatic treatments, and incubation conditions. Asymmetrical flow field-flow fractionation (AF4) is a gentle method where the separation is based on size. Here, we applied AF4 for controlled dissociation of enveloped bacteriophage phi 6. Our results indicate that AF4 can be used to assay the efficiency of the dissociation process and to purify functional subviral particles.
Subject: 1183 Plant biology, microbiology, virology
enveloped virus
bacteriophage
virus disassembly
subviral particles
field-flow fractionation
size-based separation
dsRNA virus
STRANDED-RNA BACTERIOPHAGE-PHI-6
IN-VITRO TRANSCRIPTION
LIGHT-SCATTERING
PSEUDOMONAS-SYRINGAE
PHI-6
VIRUS
PROTEIN
POLYMERASE
PARTICLES
SEGMENTS
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