Eskelin , K J & Poranen , M M 2018 , ' Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) ' , Viruses (Basel) , vol. 10 , no. 11 , 579 . https://doi.org/10.3390/v10110579
Title: | Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) |
Author: | Eskelin, Katri Johanna; Poranen, Minna Marjetta |
Contributor organization: | Doctoral Programme in Microbiology and Biotechnology Molecular and Integrative Biosciences Research Programme Faculty of Biological and Environmental Sciences Molecular and Translational Virology General Microbiology |
Date: | 2018-11 |
Language: | eng |
Number of pages: | 13 |
Belongs to series: | Viruses (Basel) |
ISSN: | 1999-4915 |
DOI: | https://doi.org/10.3390/v10110579 |
URI: | http://hdl.handle.net/10138/289800 |
Abstract: | Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer. Controlled dissociation of virions provides important information on virion composition, interactions, and stoichiometry of virion components, as well as their possible role in virus life cycles. Dissociation of viruses can be achieved by using various chemicals, enzymatic treatments, and incubation conditions. Asymmetrical flow field-flow fractionation (AF4) is a gentle method where the separation is based on size. Here, we applied AF4 for controlled dissociation of enveloped bacteriophage phi 6. Our results indicate that AF4 can be used to assay the efficiency of the dissociation process and to purify functional subviral particles. |
Subject: |
1183 Plant biology, microbiology, virology
enveloped virus bacteriophage virus disassembly subviral particles field-flow fractionation size-based separation dsRNA virus STRANDED-RNA BACTERIOPHAGE-PHI-6 IN-VITRO TRANSCRIPTION LIGHT-SCATTERING PSEUDOMONAS-SYRINGAE PHI-6 VIRUS PROTEIN POLYMERASE PARTICLES SEGMENTS |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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