3D morphometric analysis of calcified cartilage properties using micro-computed tomography

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dc.contributor University of Helsinki, Department of Physics en
dc.contributor University of Helsinki, Department of Physics en
dc.contributor.author Kauppinen, S.
dc.contributor.author Karhula, S. S.
dc.contributor.author Thevenot, J.
dc.contributor.author Ylitalo, T.
dc.contributor.author Rieppo, L.
dc.contributor.author Kestilä, I.
dc.contributor.author Haapea, M.
dc.contributor.author Hadjab, I.
dc.contributor.author Finnilä, M. A.
dc.contributor.author Quenneville, E.
dc.contributor.author Garon, M.
dc.contributor.author Gahunia, H. K.
dc.contributor.author Pritzker, K. P. H.
dc.contributor.author Buschmann, M. D.
dc.contributor.author Saarakkala, S.
dc.contributor.author Nieminen, H. J.
dc.date.accessioned 2019-01-11T08:01:01Z
dc.date.available 2019-01-11T08:01:01Z
dc.date.issued 2019-01
dc.identifier.citation Kauppinen , S , Karhula , S S , Thevenot , J , Ylitalo , T , Rieppo , L , Kestilä , I , Haapea , M , Hadjab , I , Finnilä , M A , Quenneville , E , Garon , M , Gahunia , H K , Pritzker , K P H , Buschmann , M D , Saarakkala , S & Nieminen , H J 2019 , ' 3D morphometric analysis of calcified cartilage properties using micro-computed tomography ' , Osteoarthritis and Cartilage , vol. 27 , no. 1 , pp. 172-180 . https://doi.org/10.1016/j.joca.2018.09.009 en
dc.identifier.issn 1063-4584
dc.identifier.other PURE: 121052895
dc.identifier.other PURE UUID: 1437a7cc-749c-465c-bbc3-b118ed7bcf6c
dc.identifier.other WOS: 000454115800019
dc.identifier.other Scopus: 85055877082
dc.identifier.uri http://hdl.handle.net/10138/290311
dc.description.abstract Objective: Our aim is to establish methods for quantifying morphometric properties of calcified cartilage (CC) from micro-computed tomography (mu CT). Furthermore, we evaluated the feasibility of these methods in investigating relationships between osteoarthritis (OA), tidemark surface morphology and open subchondral channels (OSCCs). Method: Samples (n = 15) used in this study were harvested from human lateral tibial plateau (n = 8). Conventional roughness and parameters assessing local 3-dimensional (3D) surface variations were used to quantify the surface morphology of the CC. Subchondral channel properties (percentage, density, size) were also calculated. As a reference, histological sections were evaluated using Histopathological osteoarthritis grading (OARSI) and thickness of CC and subchondral bone (SCB) was quantified. Results: OARSI grade correlated with a decrease in local 3D variations of the tidemark surface (amount of different surface patterns (r(s) = -0.600, P = 0.018), entropy of patterns (EP) (r(s) = -0.648, P = 0.018), homogeneity index (HI) (r(s) = 0.555, P = 0.032)) and tidemark roughness (TMR) (r(s) = -0.579, P = 0.024). Amount of different patterns (ADP) and EP associated with channel area fraction (CAF) (r(p) = 0.876, P <0.0001; r(p) = 0.665, P = 0.007, respectively) and channel density (CD) (r(p) = 0.680, P = 0.011; r(p) = 0.582, P = 0.023, respectively). TMR was associated with CAF (r(p) = 0.926, P <0.0001) and average channel size (r(p) = 0.574, P = 0.025). CC topography differed statistically significantly in early OA vs healthy samples. Conclusion: We introduced a mu-CT image method to quantify 3D CC topography and perforations through CC. CC topography was associated with OARSI grade and OSCC properties; this suggests that the established methods can detect topographical changes in tidemark and CC perforations associated with OA. (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Osteoarthritis and Cartilage
dc.rights en
dc.subject Osteoarthritis en
dc.subject Calcified cartilage en
dc.subject Tidemark en
dc.subject Morphology en
dc.subject Micro-computed tomography en
dc.subject Roughness en
dc.subject SUBCHONDRAL BONE en
dc.subject ARTICULAR-CARTILAGE en
dc.subject OSTEOARTHRITIS en
dc.subject MORPHOLOGY en
dc.subject PATHOGENESIS en
dc.subject CALCIFICATION en
dc.subject PROGRESSION en
dc.subject INITIATION en
dc.subject TIDEMARK en
dc.subject PLATE en
dc.subject 318 Medical biotechnology en
dc.subject 114 Physical sciences en
dc.title 3D morphometric analysis of calcified cartilage properties using micro-computed tomography en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1016/j.joca.2018.09.009
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl

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