Comparison of intestinal expression of the apical sodium-dependent bile acid transporter between dogs with and without chronic inflammatory enteropathy

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Giaretta , P R , Rech , R R , Guard , B C , Blake , A B , Blick , A K , Steiner , J M , Lidbury , J A , Cook , A K , Hanifeh , M , Spillmann , T , Kilpinen , S , Syrjä , P & Suchodolski , J 2018 , ' Comparison of intestinal expression of the apical sodium-dependent bile acid transporter between dogs with and without chronic inflammatory enteropathy ' , Journal of Veterinary Internal Medicine , vol. 32 , no. 6 , pp. 1918-1926 . https://doi.org/10.1111/jvim.15332

Title: Comparison of intestinal expression of the apical sodium-dependent bile acid transporter between dogs with and without chronic inflammatory enteropathy
Author: Giaretta, Paula R; Rech, Raquel R; Guard, Blake C; Blake, Amanda B; Blick, Anna K; Steiner, Jörg M.; Lidbury, Jonathan A; Cook, Audrey K; Hanifeh, Mohsen; Spillmann, Thomas; Kilpinen, Susanne; Syrjä, Pernilla; Suchodolski, Jan
Contributor: University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, Veterinary Pathology and Parasitology
Date: 2018
Language: eng
Number of pages: 9
Belongs to series: Journal of Veterinary Internal Medicine
ISSN: 1939-1676
URI: http://hdl.handle.net/10138/293040
Abstract: Background Objective Intestinal absorption of bile acids is mediated by the apical sodium-dependent bile acid transporter (ASBT). Fecal bile acid dysmetabolism has been reported in dogs with chronic inflammatory enteropathy (CIE). Characterization of ASBT distribution along the intestinal tract of control dogs and comparison to dogs with CIE. Animals Methods Twenty-four dogs with CIE and 11 control dogs. The ASBT mRNA and protein expression were assessed using RNA in situ hybridization and immunohistochemistry, respectively. The concentrations of fecal bile acids were measured by gas chromatography-mass spectrometry. The fecal microbiota dysbiosis index was assessed with a quantitative polymerase chain reaction panel. Results Conclusions and Clinical Importance In control dogs, ASBT mRNA expression was observed in enterocytes in all analyzed intestinal segments, with highest expression in the ileum. The ASBT protein expression was restricted to enterocytes in the ileum, cecum, and colon. Dogs with CIE had significantly decreased expression of ASBT protein in the ileum (P = .001), which was negatively correlated with histopathological score (rho = -0.40; P-corr = .049). Additionally, dogs with CIE had a significantly increased percentage of primary bile acids in feces compared to controls (P = .04). The fecal dysbiosis index was significantly higher in dogs with CIE than in control dogs (P = .01). These findings indicate that ileal protein expression of ASBT is downregulated in dogs with CIE. This change may be linked to the inflammatory process, intestinal dysbiosis, and fecal bile acid dysmetabolism observed in these patients.
Subject: dysbiosis
ileal bile acid transporter
inflammatory bowel disease
SLC10A2
MESSENGER-RNA EXPRESSION
BOWEL-DISEASE
ILEAL RESECTION
MALABSORPTION
DYSBIOSIS
CHOLESTYRAMINE
DIAGNOSIS
DIARRHEA
CLONING
ASBT
413 Veterinary science
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