Comparative Genomics of the Baltic Sea Toxic Cyanobacteria Nodularia spumigena UHCC 0039 and Its Response to Varying Salinity

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Teikari , J E , Hou , S , Wahlsten , M , Hess , W R & Sivonen , K 2018 , ' Comparative Genomics of the Baltic Sea Toxic Cyanobacteria Nodularia spumigena UHCC 0039 and Its Response to Varying Salinity ' , Frontiers in Microbiology , vol. 9 , 356 . https://doi.org/10.3389/fmicb.2018.00356

Title: Comparative Genomics of the Baltic Sea Toxic Cyanobacteria Nodularia spumigena UHCC 0039 and Its Response to Varying Salinity
Author: Teikari, Jonna E.; Hou, Shengwei; Wahlsten, Matti; Hess, Wolfgang R.; Sivonen, Kaarina
Contributor organization: Cyanobacteria research
Department of Microbiology
Helsinki Institute of Sustainability Science (HELSUS)
Doctoral Programme in Food Chain and Health
Doctoral Programme in Microbiology and Biotechnology
Date: 2018-03-08
Language: eng
Number of pages: 13
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
DOI: https://doi.org/10.3389/fmicb.2018.00356
URI: http://hdl.handle.net/10138/297089
Abstract: Salinity is an important abiotic factor controlling the distribution and abundance of Nodularia spumigena, the dominating diazotrophic and toxic phototroph, in the brackish water cyanobacterial blooms of the Baltic Sea. To expand the available genomic information for brackish water cyanobacteria, we sequenced the isolate Nodularia spurn/germ UHCC 0039 using an Illumina-SMRT hybrid sequencing approach, revealing a chromosome of 5,294,286 base pairs (bp) and a single plasmid of 92,326 bp. Comparative genomics in Nostocales showed pronounced genetic similarity among Nodularia spumigena strains evidencing their short evolutionary history. The studied Baltic Sea strains share similar sets of CRISPR-Cas cassettes and a higher number of insertion sequence (IS) elements compared to Nodularia spumigena CENA596 isolated from a shrimp production pond in Brazil. Nodularia spumigena UHCC 0039 proliferated similarly at three tested salinities, whereas the lack of salt inhibited its growth and triggered transcriptome remodeling, including the up-regulation of five sigma factors and the down-regulation of two other sigma factors, one of which is specific for strain UHCC 0039. Down-regulated genes additionally included a large genetic region for the synthesis of two yet unidentified natural products. Our results indicate a remarkable plasticity of the Nodularia salinity acclimation, and thus salinity strongly impacts the intensity and distribution of cyanobacterial blooms in the Baltic Sea.
Subject: Baltic Sea
cyanobacteria
Nodularia spumigena
comparative genomics
RNA sequencing
salinity
SP STRAIN PCC-6803
DIFFERENTIAL EXPRESSION ANALYSIS
SYNECHOCYSTIS SP PCC-6803
SP PCC 6803
CRISPR-CAS SYSTEMS
SIGMA-FACTOR SIGE
SALT-STRESS
MICROCYSTIS-AERUGINOSA
PHOSPHATE-SYNTHASE
GENE-EXPRESSION
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess


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