Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

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Daly , P , Lopez , S C , Peng , M , Lancefield , C S , Purvine , S O , Kim , Y-M , Zink , E M , Dohnalkova , A , Singan , V R , Lipzen , A , Dilworth , D , Wang , M , Ng , V , Robinson , E , Orr , G , Baker , S E , Bruijnincx , P C A , Hilden , K S , Grigoriev , I V , Mäkelä , M R & de Vries , R P 2018 , ' Dichomitus squalens partially tailors its molecular responses to the composition of solid wood ' , Environmental Microbiology , vol. 20 , no. 11 , pp. 4141-4156 . https://doi.org/10.1111/1462-2920.14416

Title: Dichomitus squalens partially tailors its molecular responses to the composition of solid wood
Author: Daly, Paul; Lopez, Sara Casado; Peng, Mao; Lancefield, Christopher S.; Purvine, Samuel O.; Kim, Young-Mo; Zink, Erika M.; Dohnalkova, Alice; Singan, Vasanth R.; Lipzen, Anna; Dilworth, David; Wang, Mei; Ng, Vivian; Robinson, Errol; Orr, Galya; Baker, Scott E.; Bruijnincx, Pieter C. A.; Hilden, Kristiina S.; Grigoriev, Igor V.; Mäkelä, Miia R.; de Vries, Ronald P.
Contributor organization: Department of Microbiology
Helsinki Institute of Sustainability Science (HELSUS)
Fungal Genetics and Biotechnology
Date: 2018-11
Language: eng
Number of pages: 16
Belongs to series: Environmental Microbiology
ISSN: 1462-2912
DOI: https://doi.org/10.1111/1462-2920.14416
URI: http://hdl.handle.net/10138/297704
Abstract: White-rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed-wood forests containing both soft- and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi-natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D-NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G-lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.
Subject: WHITE-ROT FUNGI
PLANT BIOMASS
PHANEROCHAETE-CHRYSOSPORIUM
LIQUID-CHROMATOGRAPHY
AROMATIC-COMPOUNDS
ASPERGILLUS-NIGER
GENE-EXPRESSION
ACCURATE MASS
LIGNIN
DEGRADATION
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: draft


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