Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

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dc.contributor.author Daly, Paul
dc.contributor.author Lopez, Sara Casado
dc.contributor.author Peng, Mao
dc.contributor.author Lancefield, Christopher S.
dc.contributor.author Purvine, Samuel O.
dc.contributor.author Kim, Young-Mo
dc.contributor.author Zink, Erika M.
dc.contributor.author Dohnalkova, Alice
dc.contributor.author Singan, Vasanth R.
dc.contributor.author Lipzen, Anna
dc.contributor.author Dilworth, David
dc.contributor.author Wang, Mei
dc.contributor.author Ng, Vivian
dc.contributor.author Robinson, Errol
dc.contributor.author Orr, Galya
dc.contributor.author Baker, Scott E.
dc.contributor.author Bruijnincx, Pieter C. A.
dc.contributor.author Hilden, Kristiina S.
dc.contributor.author Grigoriev, Igor V.
dc.contributor.author Mäkelä, Miia R.
dc.contributor.author de Vries, Ronald P.
dc.date.accessioned 2019-01-23T10:47:01Z
dc.date.available 2019-01-23T10:47:01Z
dc.date.issued 2018-11
dc.identifier.citation Daly , P , Lopez , S C , Peng , M , Lancefield , C S , Purvine , S O , Kim , Y-M , Zink , E M , Dohnalkova , A , Singan , V R , Lipzen , A , Dilworth , D , Wang , M , Ng , V , Robinson , E , Orr , G , Baker , S E , Bruijnincx , P C A , Hilden , K S , Grigoriev , I V , Mäkelä , M R & de Vries , R P 2018 , ' Dichomitus squalens partially tailors its molecular responses to the composition of solid wood ' , Environmental Microbiology , vol. 20 , no. 11 , pp. 4141-4156 . https://doi.org/10.1111/1462-2920.14416
dc.identifier.other PURE: 121543211
dc.identifier.other PURE UUID: da6106ba-47ef-4c74-99f0-e01f389951b0
dc.identifier.other WOS: 000450357400018
dc.identifier.other Scopus: 85055283312
dc.identifier.other ORCID: /0000-0003-0771-2329/work/65310674
dc.identifier.uri http://hdl.handle.net/10138/297704
dc.description.abstract White-rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed-wood forests containing both soft- and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi-natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D-NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G-lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof Environmental Microbiology
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject WHITE-ROT FUNGI
dc.subject PLANT BIOMASS
dc.subject PHANEROCHAETE-CHRYSOSPORIUM
dc.subject LIQUID-CHROMATOGRAPHY
dc.subject AROMATIC-COMPOUNDS
dc.subject ASPERGILLUS-NIGER
dc.subject GENE-EXPRESSION
dc.subject ACCURATE MASS
dc.subject LIGNIN
dc.subject DEGRADATION
dc.subject 1183 Plant biology, microbiology, virology
dc.title Dichomitus squalens partially tailors its molecular responses to the composition of solid wood en
dc.type Article
dc.contributor.organization Department of Microbiology
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Fungal Genetics and Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1111/1462-2920.14416
dc.relation.issn 1462-2912
dc.rights.accesslevel openAccess
dc.type.version draft

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