Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats

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http://hdl.handle.net/10138/298055

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Buntin , N , de Vos , W M & Hongpattarakere , T 2017 , ' Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats ' , Applied Microbiology and Biotechnology , vol. 101 , no. 20 , pp. 7663-7674 . https://doi.org/10.1007/s00253-017-8482-3

Title: Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats
Author: Buntin, Nirunya; de Vos, Willem M.; Hongpattarakere, Tipparat
Contributor: University of Helsinki, Medicum
Date: 2017-10
Language: eng
Number of pages: 12
Belongs to series: Applied Microbiology and Biotechnology
ISSN: 0175-7598
URI: http://hdl.handle.net/10138/298055
Abstract: The adhesion ability to mucin varied greatly among 18 Lactobacillus plantarum isolates depending on their isolation habitats. Such ability remained at high level even though they were sequentially exposed to the gastrointestinal (GI) stresses. The majority of L. plantarum isolated from shrimp intestine and about half of food isolates exhibited adhesion ability (51.06-55.04%) about the same as the well-known adhesive L. plantarum 299v. Interestingly, five infant isolates of CIF17A2, CIF17A4, CIF17A5, CIF17AN2, and CIF17AN8 exhibited extremely high adhesion ranging from 62.69 to 72.06%. Such highly adhesive property correlating to distinctively high cell surface hydrophobicity was significantly weaken after pretreatment with LiCl and guanidine-HCl confirming the entailment of protein moiety. Regarding the draft genome information, all molecular structures of major cell wall-anchored proteins involved in the adhesion based on L. plantarum WCSF1, including lp_0964, lp_1643, lp_3114, lp_2486, lp_3127, and lp_3059 orthologues were detected in all isolates. Exceptionally, the gene-trait matching between yeast agglutination assay and the relevant mannose-specific adhesin (lp_1229) encoding gene confirmed the Msa absence in five infant isolates expressed distinctively high adhesion. Interestingly, the predicted flagellin encoding genes (fliC) firstly revealed in lp_1643, lp_2486, and lp_3114 orthologues may potentially contribute to such highly adhesive property of these isolates.
Subject: Lactobacillus fliC
Mucin-adhesion mechanism
Mannose-specific adhesion
Mucus-binding protein
Gene-trait matching
Competitive adhesion
COLLAGEN-BINDING PROTEIN
LACTIC-ACID BACTERIA
PROBIOTIC PROPERTIES
INTESTINAL MUCUS
IMMUNE-RESPONSE
GENOME SEQUENCE
STRAINS
DOMAIN
TRACT
IDENTIFICATION
3111 Biomedicine
318 Medical biotechnology
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