Buntin , N , de Vos , W M & Hongpattarakere , T 2017 , ' Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats ' , Applied Microbiology and Biotechnology , vol. 101 , no. 20 , pp. 7663-7674 . https://doi.org/10.1007/s00253-017-8482-3
Title: | Variation of mucin adhesion, cell surface characteristics, and molecular mechanisms among Lactobacillus plantarum isolated from different habitats |
Author: | Buntin, Nirunya; de Vos, Willem M.; Hongpattarakere, Tipparat |
Contributor organization: | Medicum Willem Meindert Vos de / Principal Investigator Veterinary Biosciences Veterinary Microbiology and Epidemiology Immunobiology Research Program Research Programs Unit de Vos & Salonen group University of Helsinki Department of Bacteriology and Immunology |
Date: | 2017-10 |
Language: | eng |
Number of pages: | 12 |
Belongs to series: | Applied Microbiology and Biotechnology |
ISSN: | 0175-7598 |
DOI: | https://doi.org/10.1007/s00253-017-8482-3 |
URI: | http://hdl.handle.net/10138/298055 |
Abstract: | The adhesion ability to mucin varied greatly among 18 Lactobacillus plantarum isolates depending on their isolation habitats. Such ability remained at high level even though they were sequentially exposed to the gastrointestinal (GI) stresses. The majority of L. plantarum isolated from shrimp intestine and about half of food isolates exhibited adhesion ability (51.06-55.04%) about the same as the well-known adhesive L. plantarum 299v. Interestingly, five infant isolates of CIF17A2, CIF17A4, CIF17A5, CIF17AN2, and CIF17AN8 exhibited extremely high adhesion ranging from 62.69 to 72.06%. Such highly adhesive property correlating to distinctively high cell surface hydrophobicity was significantly weaken after pretreatment with LiCl and guanidine-HCl confirming the entailment of protein moiety. Regarding the draft genome information, all molecular structures of major cell wall-anchored proteins involved in the adhesion based on L. plantarum WCSF1, including lp_0964, lp_1643, lp_3114, lp_2486, lp_3127, and lp_3059 orthologues were detected in all isolates. Exceptionally, the gene-trait matching between yeast agglutination assay and the relevant mannose-specific adhesin (lp_1229) encoding gene confirmed the Msa absence in five infant isolates expressed distinctively high adhesion. Interestingly, the predicted flagellin encoding genes (fliC) firstly revealed in lp_1643, lp_2486, and lp_3114 orthologues may potentially contribute to such highly adhesive property of these isolates. |
Subject: |
Lactobacillus fliC
Mucin-adhesion mechanism Mannose-specific adhesion Mucus-binding protein Gene-trait matching Competitive adhesion COLLAGEN-BINDING PROTEIN LACTIC-ACID BACTERIA PROBIOTIC PROPERTIES INTESTINAL MUCUS IMMUNE-RESPONSE GENOME SEQUENCE STRAINS DOMAIN TRACT IDENTIFICATION 3111 Biomedicine 318 Medical biotechnology |
Peer reviewed: | Yes |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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