Alcohol intake in two different mouse drinking models after recovery from the lipopolysaccharide-induced sickness reaction

Show full item record



Permalink

http://hdl.handle.net/10138/298149

Citation

Lainiola , M & Linden , A-M 2017 , ' Alcohol intake in two different mouse drinking models after recovery from the lipopolysaccharide-induced sickness reaction ' , Alcohol (New York) , vol. 65 , pp. 1-10 . https://doi.org/10.1016/j.alcohol.2017.06.002

Title: Alcohol intake in two different mouse drinking models after recovery from the lipopolysaccharide-induced sickness reaction
Author: Lainiola, Mira; Linden, Anni-Maija
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2017-12
Language: eng
Number of pages: 10
Belongs to series: Alcohol (New York)
ISSN: 0741-8329
URI: http://hdl.handle.net/10138/298149
Abstract: Neuroinflammation may play an important role in the development of alcohol addiction. Recent pre-clinical reports suggest that enhanced innate immune system signaling increases consumption of alcohol. Our aim was to study whether consequences of lipopolysaccharide (LPS)-induced sickness reaction increase long-term alcohol intake. Adult male C57BL/6j mice, housed in individually ventilated cages, were injected with LPS intraperitoneally (i.p.) and allowed to recover from an acute sickness reaction for 1 week before analysis of their alcohol intake in two different drinking models. Effects of LPS challenge were tested in a continuous two-bottle free choice test with increasing concentrations of alcohol and in a drinking in the dark (DID) binge model. In addition, the effect of repeatedly administered LPS during abstinence periods between binge drinking was analyzed in the DID model. In addition, the DID model was used to study the effects of the microglia inhibitor minocycline (50 mg/kg/day, 4 days) and purinergic P2X7 receptor antagonist Brilliant Blue G (75 mg/kg/day, 7 days) on alcohol intake. In contrast to previous findings, pretreatment with a 1-mg/kg dose of LPS did not significantly increase ethanol consumption in the continuous two-bottle choice test. As a novel finding, we report that increasing the LPS dose to 1.5 mg/kg reduced consumption of 18 and 21% (v/v) ethanol. In the DID model, pretreatment with LPS (0.2-1.5 mg/kg) did not significantly alter 15% or 20% ethanol consumption. Neither did repeated LPS injections affect binge alcohol drinking. Minocycline reduced alcohol, but also water, intake regardless of LPS pretreatment. No data on effects of P2X7 antagonists on alcohol consumption have been previously published; therefore, we report here that subchronic Brilliant Blue G had no effect on alcohol intake in the DID model. As a conclusion, further studies are needed to validate this LPS model of the interaction between immune system activation and alcohol consumption. (C) 2017 Elsevier Inc. All rights reserved.
Subject: Neuroinflammation
Lipopolysaccharide
Alcohol consumption
Individually ventilated cages
Mouse
HIGH ETHANOL DRINKING
MALE C57BL/6J MICE
INTESTINAL PERMEABILITY
MOOD DISORDERS
BINGE-LIKE
CONSUMPTION
RECEPTOR
INFLAMMATION
ADDICTION
BRAIN
317 Pharmacy
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S0741832917305165_main.pdf 2.014Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record