Lauren , E , Tigistu-Sahle , F , Valkonen , S , Westberg , M , Valkeajarvi , A , Eronen , J , Siljander , P R-M , Pettila , V , Kakela , R , Laitinen , S & Kerkela , E 2018 , ' Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage ' , Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids , vol. 1863 , no. 1 , pp. 1-8 . https://doi.org/10.1016/j.bbalip.2017.09.012
Title: | Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage |
Author: | Lauren, Eva; Tigistu-Sahle, Feven; Valkonen, Sami; Westberg, Melissa; Valkeajarvi, Anne; Eronen, Juha; Siljander, Pia R-M; Pettila, Ville; Kakela, Reijo; Laitinen, Saara; Kerkela, Erja |
Contributor organization: | Biosciences Physiology and Neuroscience (-2020) University of Helsinki Faculty of Pharmacy Extracellular Vesicles Division of Pharmaceutical Biosciences Biochemistry and Biotechnology Clinicum Department of Diagnostics and Therapeutics Anestesiologian yksikkö HUS Perioperative, Intensive Care and Pain Medicine Functional Lipidomics Group |
Date: | 2018-01 |
Language: | eng |
Number of pages: | 8 |
Belongs to series: | Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids |
ISSN: | 1388-1981 |
DOI: | https://doi.org/10.1016/j.bbalip.2017.09.012 |
URI: | http://hdl.handle.net/10138/298176 |
Abstract: | Red blood cells (RBCs) are stored up to 35-42 days at 2-6 degrees C in blood banks. During storage, the RBC membrane is challenged by energy depletion, decreasing pH, altered cation homeostasis, and oxidative stress, leading to several biochemical and morphological changes in RBCs and to shedding of extracellular vesicles (EVs) into the storage medium. These changes are collectively known as RBC storage lesions. EVs accumulate in stored RBC concentrates and are, thus, transfused into patients. The potency of EVs as bioactive effectors is largely acknowledged, and EVs in RBC concentrates are suspected to mediate some adverse effects of transfusion. Several studies have shown accumulation of lipid raft associated proteins in RBC EVs during storage, whereas a comprehensive phospholipidomic study on RBCs and corresponding EVs during the clinical storage period is lacking. Our mass spectrometric and chromatographic study shows that RBCs maintain their major phospholipid (PL) content well during storage despite abundant vesiculation. The phospholipidomes were largely similar between RBCs and EVs. No accumulation of raft lipids in EVs was seen, suggesting that the primary mechanism of RBC vesiculation during storage might not be raft-based. Nonetheless, a slight tendency of EV PLs for shorter acyl chains was observed. |
Subject: |
Stored red blood cells
Storage lesion Extracellular vesicles Phospholipids Mass spectrometry FATTY-ACIDS SUPERLATTICE MODEL HUMAN-ERYTHROCYTES MASS-SPECTROMETRY IN-VITRO MEMBRANE MICROPARTICLES MICROVESICLES VESICULATION EXOSOMES 1182 Biochemistry, cell and molecular biology |
Peer reviewed: | Yes |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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