Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage

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http://hdl.handle.net/10138/298176

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Lauren , E , Tigistu-Sahle , F , Valkonen , S , Westberg , M , Valkeajarvi , A , Eronen , J , Siljander , P R-M , Pettila , V , Kakela , R , Laitinen , S & Kerkela , E 2018 , ' Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage ' , Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids , vol. 1863 , no. 1 , pp. 1-8 . https://doi.org/10.1016/j.bbalip.2017.09.012

Title: Phospholipid composition of packed red blood cells and that of extracellular vesicles show a high resemblance and stability during storage
Author: Lauren, Eva; Tigistu-Sahle, Feven; Valkonen, Sami; Westberg, Melissa; Valkeajarvi, Anne; Eronen, Juha; Siljander, Pia R-M; Pettila, Ville; Kakela, Reijo; Laitinen, Saara; Kerkela, Erja
Other contributor: University of Helsinki, Biosciences
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Extracellular Vesicles
University of Helsinki, Clinicum
University of Helsinki, Biosciences











Date: 2018-01
Language: eng
Number of pages: 8
Belongs to series: Biochimica and Biophysica Acta. Molecular and Cell Biology of Lipids
ISSN: 1388-1981
DOI: https://doi.org/10.1016/j.bbalip.2017.09.012
URI: http://hdl.handle.net/10138/298176
Abstract: Red blood cells (RBCs) are stored up to 35-42 days at 2-6 degrees C in blood banks. During storage, the RBC membrane is challenged by energy depletion, decreasing pH, altered cation homeostasis, and oxidative stress, leading to several biochemical and morphological changes in RBCs and to shedding of extracellular vesicles (EVs) into the storage medium. These changes are collectively known as RBC storage lesions. EVs accumulate in stored RBC concentrates and are, thus, transfused into patients. The potency of EVs as bioactive effectors is largely acknowledged, and EVs in RBC concentrates are suspected to mediate some adverse effects of transfusion. Several studies have shown accumulation of lipid raft associated proteins in RBC EVs during storage, whereas a comprehensive phospholipidomic study on RBCs and corresponding EVs during the clinical storage period is lacking. Our mass spectrometric and chromatographic study shows that RBCs maintain their major phospholipid (PL) content well during storage despite abundant vesiculation. The phospholipidomes were largely similar between RBCs and EVs. No accumulation of raft lipids in EVs was seen, suggesting that the primary mechanism of RBC vesiculation during storage might not be raft-based. Nonetheless, a slight tendency of EV PLs for shorter acyl chains was observed.
Subject: Stored red blood cells
Storage lesion
Extracellular vesicles
Phospholipids
Mass spectrometry
FATTY-ACIDS
SUPERLATTICE MODEL
HUMAN-ERYTHROCYTES
MASS-SPECTROMETRY
IN-VITRO
MEMBRANE
MICROPARTICLES
MICROVESICLES
VESICULATION
EXOSOMES
1182 Biochemistry, cell and molecular biology
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