Specific status of Echinococcus canadensis (Cestoda Taeniidae) inferred from nuclear and mitochondrial gene sequences

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Yanagida , T , Lavikainen , A , Hoberg , E P , Konyaev , S , Ito , A , Sato , M O , Zaikov , V A , Beckmen , K & Nakao , M 2017 , ' Specific status of Echinococcus canadensis (Cestoda Taeniidae) inferred from nuclear and mitochondrial gene sequences ' , International Journal for Parasitology , vol. 47 , no. 14 , pp. 971-979 . https://doi.org/10.1016/j.ijpara.2017.07.001

Title: Specific status of Echinococcus canadensis (Cestoda Taeniidae) inferred from nuclear and mitochondrial gene sequences
Author: Yanagida, Tetsuya; Lavikainen, Antti; Hoberg, Eric P.; Konyaev, Sergey; Ito, Akira; Sato, Marcello Otake; Zaikov, Vladimir A.; Beckmen, Kimberlee; Nakao, Minoru
Contributor: University of Helsinki, Medicum
Date: 2017-12
Language: eng
Number of pages: 9
Belongs to series: International Journal for Parasitology
ISSN: 0020-7519
URI: http://hdl.handle.net/10138/298234
Abstract: The specific status of Echinococcus canadensis has long been controversial, mainly because it consists of the mitochondrial lineages G6, G7, G8 and G10 with different host affinity: G6 (camel strain) and G7 (pig strain) with domestic cycles and G8 (cervid strain) and G10 (Fennoscandian cervid strain) with sylvatic or semi-domestic cycles. There is an argument whether the mitochondria] lineages should be recognised as separate species which correspond to the biological or epidemiological aggregation. In the present study, the specific status of E. canadensis was investigated using mitochondrial DNA and single copy nuclear DNA markers. Nucleotide sequences of complete mitochondrial cytochrome c oxidase subunit 1 (cox1) and partial nuclear phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold) were determined for 48 isolates of E. canadensis collected from different hosts in a wide range of regions. The mitochondrial phylogeny of cox1 showed that all the isolates were clearly divided into three clades corresponding to G6/G7, G8 and G10. Five and three alleles were confirmed at pepck and pold loci, respectively. These alleles were generally divided into two groups corresponding to G6/G7 or G8 and G10. However, allele sharing was confirmed among individuals belonging to different lineages. The allele sharing occurred primarily in regions where different mitochondrial DNA lineages were found in sympatry. The resultant nuclear mitochondrial discordance suggests the genetic exchangeability among E. canadensis isolates belonging to different lineages. An apparently mosaic parasite fauna that reflects faunal mixing due to natural and anthropogenic disturbance, including introductions and invasion, precludes us from designating each of G6/G7, G8 and G10 into a different species. (C) 2017 Australian Society for Parasitology.
Subject: Echinococcus canadensis
coxl
pold
pepck
Nuclear-mitochondrial discordance
PHYLOGENETIC-RELATIONSHIPS
CYSTIC ECHINOCOCCOSIS
CERVID STRAIN
GRANULOSUS
GENOTYPES
ASIATICA
SAGINATA
DOMESTICATION
HYBRIDIZATION
INTERMEDIUS
3111 Biomedicine
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