Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults

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Jäsberg , H , Tervahartiala , T , Sorsa , T , Söderling , E & Haukioja , A 2018 , ' Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults ' , Archives of Oral Biology , vol. 85 , pp. 58-63 . https://doi.org/10.1016/j.archoralbio.2017.10.003

Title: Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults
Author: Jäsberg, Heli; Tervahartiala, Taina; Sorsa, Timo; Söderling, Eva; Haukioja, Anna
Contributor: University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2018-01
Language: eng
Number of pages: 6
Belongs to series: Archives of Oral Biology
ISSN: 0003-9969
URI: http://hdl.handle.net/10138/298241
Abstract: Objective: To study the effect of orally administered Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG on the salivary levels of Matrix Metalloproteinases (MMP)-8, MMP-9 and of Tissue Inhibitor of Metalloproteinases (TIMP)-1 in healthy adults. Furthermore, the correlations between MMP-8, MMP-9 and TIMP-1 and plaque and gingival indices, salivary mutans streptococci and lactobacilli counts, and stimulated saliva secretion rate were analysed. Design: The salivary samples originated from a randomized controlled trial where healthy student volunteers consumed probiotic or placebo lozenges twice a day for four weeks. The saliva samples were collected and clinical parameters measured at the baseline and at the end of the original study. For this study, the salivary levels of MMP-8, MMP-9 and TIMP-1 were analysed with immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Results: In the probiotic group (n = 29), salivary MMP-9 levels increased (p <0.01) and TIMP-1 levels decreased (p <0.01) significantly during the intervention. Furthermore, MMP-9/TIMP-1 ratio differed significantly from the baseline level (p <0.01). These changes were not observed in the control group (n = 31). In the whole data, salivary MMP-9 and gingival index correlated (r = 0.260, p <0.05 at baseline and r = 0.354, p <0.01 at the end of the study). Intergroup differences or correlations with other clinical parameters were not found. Probiotic consumption did not affect the saliva flow rate. Conclusions: Increased MMP-9 and decreased TIMP-1 levels in saliva may indicate that probiotics have immunomodulatory effects in the oral cavity. Furthermore, increased salivary MMP-9 levels may be an indication of the defensive potential of matrix metalloproteinases.
Subject: Probiotic
Lactobacillus rhamnosus GG
Blfidobacterium animalis subsp lactis BB-12
Matrix metalloproteinase (MMP)
Tissue inhibitor of metalloproteinases (TIMP)
Saliva
SUBSP LACTIS BB-12
LACTOBACILLUS-RHAMNOSUS GG
RANDOMIZED CLINICAL-TRIAL
GINGIVAL CREVICULAR FLUID
MUTANS STREPTOCOCCI
IN-VITRO
PORPHYROMONAS-GINGIVALIS
CHRONIC PERIODONTITIS
PRESCHOOL-CHILDREN
ORAL COLONIZATION
313 Dentistry
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