Construction and characterization of synthetic bacterial community for experimental ecology and evolution

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Cairns , J , Jokela , R , Hultman , J , Tamminen , M , Virta , M & Hiltunen , T 2018 , ' Construction and characterization of synthetic bacterial community for experimental ecology and evolution ' , Frontiers in Genetics , vol. 9 , 312 . https://doi.org/10.3389/fgene.2018.00312

Title: Construction and characterization of synthetic bacterial community for experimental ecology and evolution
Author: Cairns, Johannes; Jokela, Roosa; Hultman, Jenni; Tamminen, Manu; Virta, Marko; Hiltunen, Teppo
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
University of Helsinki, University of Turku
University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
University of Helsinki, Department of Microbiology
Date: 2018-08-14
Language: eng
Number of pages: 12
Belongs to series: Frontiers in Genetics
ISSN: 1664-8021
URI: http://hdl.handle.net/10138/298346
Abstract: Experimental microbial ecology and evolution have yielded foundational insights into ecological and evolutionary processes using simple microcosm setups and phenotypic assays with one- or two-species model systems. The fields are now increasingly incorporating more complex systems and exploration of the molecular basis of observations. For this purpose, simplified, manageable and well-defined multispecies model systems are required that can be easily investigated using culturing and high-throughput sequencing approaches, bridging the gap between simpler and more complex synthetic or natural systems. Here we address this need by constructing a completely synthetic 33 bacterial strain community that can be cultured in simple laboratory conditions. We provide whole-genome data for all the strains as well as metadata about genomic features and phenotypic traits that allow resolving individual strains by amplicon sequencing and facilitate a variety of envisioned mechanistic studies. We further show that a large proportion of the strains exhibit coexistence in co-culture over serial transfer for 48 days in the absence of any experimental manipulation to maintain diversity. The constructed bacterial community can be a valuable resource in future experimental work.
Subject: 1181 Ecology, evolutionary biology
microbial community
model system
synthetic ecology
experimental evolution
whole-genome sequencing
MICROCOSM EXPERIMENTS
SPECIES INTERACTIONS
LIMITED RELEVANCE
ECOSYSTEM ECOLOGY
ESCHERICHIA-COLI
READ ALIGNMENT
SEARCH TOOL
IN-SITU
SYSTEM
COOPERATION
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