Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri

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Vesth , T C , Nybo , J L , Theobald , S , Frisvad , J C , Larsen , T O , Nielsen , K F , Hoof , J B , Brandl , J , Salamov , A , Riley , R , Gladden , J M , Phatale , P , Nielsen , M T , Lyhne , E K , Kogle , M E , Strasser , K , McDonnell , E , Barry , K , Clum , A , Chen , C , LaButti , K , Haridas , S , Nolan , M , Sandor , L , Kuo , A , Hainaut , M , Drula , E , Tsang , A , Magnuson , J K , Henrissat , B , Wiebenga , A , Simmons , B A , Makela , M R , de Vries , R P , Grigoriev , I V , Mortensen , U H , Baker , S E & Andersen , M R 2018 , ' Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri ' , Nature Genetics , vol. 50 , no. 12 , pp. 1688-1695 . https://doi.org/10.1038/s41588-018-0246-1

Title: Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri
Author: Vesth, Tammi C.; Nybo, Jane L.; Theobald, Sebastian; Frisvad, Jens C.; Larsen, Thomas O.; Nielsen, Kristian F.; Hoof, Jakob B.; Brandl, Julian; Salamov, Asaf; Riley, Robert; Gladden, John M.; Phatale, Pallavi; Nielsen, Morten T.; Lyhne, Ellen K.; Kogle, Martin E.; Strasser, Kimchi; McDonnell, Erin; Barry, Kerrie; Clum, Alicia; Chen, Cindy; LaButti, Kurt; Haridas, Sajeet; Nolan, Matt; Sandor, Laura; Kuo, Alan; Hainaut, Matthieu; Drula, Elodie; Tsang, Adrian; Magnuson, Jon K.; Henrissat, Bernard; Wiebenga, Ad; Simmons, Blake A.; Makela, Miia R.; de Vries, Ronald P.; Grigoriev, Igor V.; Mortensen, Uffe H.; Baker, Scott E.; Andersen, Mikael R.
Contributor: University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
Date: 2018-12
Language: eng
Belongs to series: Nature Genetics
ISSN: 1061-4036
URI: http://hdl.handle.net/10138/298357
Abstract: Aspergillus section Nigri comprises filamentous fungi relevant to biomedicine, bioenergy, health, and biotechnology. To learn more about what genetically sets these species apart, as well as about potential applications in biotechnology and biomedicine, we sequenced 23 genomes de novo, forming a full genome compendium for the section (26 species), as well as 6 Aspergillus niger isolates. This allowed us to quantify both inter-and intraspecies genomic variation. We further predicted 17,903 carbohydrateactive enzymes and 2,717 secondary metabolite gene clusters, which we condensed into 455 distinct families corresponding to compound classes, 49% of which are only found in single species. We performed metabolomics and genetic engineering to correlate genotypes to phenotypes, as demonstrated for the metabolite aurasperone, and by heterologous transfer of citrate production to Aspergillus nidulans. Experimental and computational analyses showed that both secondary metabolism and regulation are key factors that are significant in the delineation of Aspergillus species.
Subject: OCHRATOXIN-A
NIGER
DEREPLICATION
EXTRACTION
PREDICTION
ALIGNMENT
PATHWAY
FUNGI
GENUS
1184 Genetics, developmental biology, physiology
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