Mechanical stretch induced transcriptomic profiles in cardiac myocytes

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Rysä , J , Tokola , H & Ruskoaho , H J 2018 , ' Mechanical stretch induced transcriptomic profiles in cardiac myocytes ' , Scientific Reports , vol. 8 , no. 1 , 4733 .

Title: Mechanical stretch induced transcriptomic profiles in cardiac myocytes
Author: Rysä, Jaana; Tokola, Heikki; Ruskoaho, Heikki Juhani
Contributor organization: Drug Research Program
Regenerative pharmacology group
Division of Pharmacology and Pharmacotherapy
Date: 2018-01
Language: eng
Number of pages: 14
Belongs to series: Scientific Reports
ISSN: 2045-2322
Abstract: Mechanical forces are able to activate hypertrophic growth of cardiomyocytes in the overloaded myocardium. However, the transcriptional profiles triggered by mechanical stretch in cardiac myocytes are not fully understood. Here, we performed the first genome-wide time series study of gene expression changes in stretched cultured neonatal rat ventricular myocytes (NRVM)s, resulting in 205, 579, 737, 621, and 1542 differentially expressed (> 2-fold, P < 0.05) genes in response to 1, 4, 12, 24, and 48 hours of cyclic mechanical stretch. We used Ingenuity Pathway Analysis to predict functional pathways and upstream regulators of differentially expressed genes in order to identify regulatory networks that may lead to mechanical stretch induced hypertrophic growth of cardiomyocytes. We also performed micro (miRNA) expression profiling of stretched NRVMs, and identified that a total of 8 and 87 miRNAs were significantly (P < 0.05) altered by 1-12 and 24-48 hours of mechanical stretch, respectively. Finally, through integration of miRNA and mRNA data, we predicted the miRNAs that regulate mRNAs potentially leading to the hypertrophic growth induced by mechanical stretch. These analyses predicted nuclear factor-like 2 (Nrf2) and interferon regulatory transcription factors as well as the let-7 family of miRNAs as playing roles in the regulation of stretch-regulated genes in cardiomyocytes.
Subject: 3111 Biomedicine
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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