Mechanical stretch induced transcriptomic profiles in cardiac myocytes

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dc.contributor University of Helsinki, Drug Research Program en
dc.contributor.author Rysä, Jaana
dc.contributor.author Tokola, Heikki
dc.contributor.author Ruskoaho, Heikki Juhani
dc.date.accessioned 2019-01-30T14:55:02Z
dc.date.available 2019-01-30T14:55:02Z
dc.date.issued 2018-01
dc.identifier.citation Rysä , J , Tokola , H & Ruskoaho , H J 2018 , ' Mechanical stretch induced transcriptomic profiles in cardiac myocytes ' , Scientific Reports , vol. 8 , no. 1 , 4733 . https://doi.org/10.1038/s41598-018-23042-w en
dc.identifier.issn 2045-2322
dc.identifier.other PURE: 120421301
dc.identifier.other PURE UUID: 41f4ebde-e729-4585-b47b-d8eb24678e1e
dc.identifier.other Scopus: 85044338672
dc.identifier.other WOS: 000427589100010
dc.identifier.other ORCID: /0000-0001-8971-1359/work/53517111
dc.identifier.uri http://hdl.handle.net/10138/298507
dc.description.abstract Mechanical forces are able to activate hypertrophic growth of cardiomyocytes in the overloaded myocardium. However, the transcriptional profiles triggered by mechanical stretch in cardiac myocytes are not fully understood. Here, we performed the first genome-wide time series study of gene expression changes in stretched cultured neonatal rat ventricular myocytes (NRVM)s, resulting in 205, 579, 737, 621, and 1542 differentially expressed (> 2-fold, P < 0.05) genes in response to 1, 4, 12, 24, and 48 hours of cyclic mechanical stretch. We used Ingenuity Pathway Analysis to predict functional pathways and upstream regulators of differentially expressed genes in order to identify regulatory networks that may lead to mechanical stretch induced hypertrophic growth of cardiomyocytes. We also performed micro (miRNA) expression profiling of stretched NRVMs, and identified that a total of 8 and 87 miRNAs were significantly (P < 0.05) altered by 1-12 and 24-48 hours of mechanical stretch, respectively. Finally, through integration of miRNA and mRNA data, we predicted the miRNAs that regulate mRNAs potentially leading to the hypertrophic growth induced by mechanical stretch. These analyses predicted nuclear factor-like 2 (Nrf2) and interferon regulatory transcription factors as well as the let-7 family of miRNAs as playing roles in the regulation of stretch-regulated genes in cardiomyocytes. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights en
dc.subject 3111 Biomedicine en
dc.subject 317 Pharmacy en
dc.title Mechanical stretch induced transcriptomic profiles in cardiac myocytes en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1038/s41598-018-23042-w
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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