Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis

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Jensen , C T , Ahsberg , J , Sommarin , M N E , Strid , T , Somasundaram , R , Okuyama , K , Ungerback , J , Kupari , J , Airaksinen , M S , Lang , S , Bryder , D , Soneji , S , Karlsson , G & Sigvardsson , M 2018 , ' Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis ' , Journal of Experimental Medicine , vol. 215 , no. 7 , pp. 1947-1963 . https://doi.org/10.1084/jem.20171384

Title: Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
Author: Jensen, Christina T.; Ahsberg, Josefine; Sommarin, Mikael N. E.; Strid, Tobias; Somasundaram, Rajesh; Okuyama, Kazuki; Ungerback, Jonas; Kupari, Jussi; Airaksinen, Matti S.; Lang, Stefan; Bryder, David; Soneji, Shamit; Karlsson, Goran; Sigvardsson, Mikael
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2018-07
Language: eng
Number of pages: 17
Belongs to series: Journal of Experimental Medicine
ISSN: 0022-1007
URI: http://hdl.handle.net/10138/298563
Abstract: To understand the developmental trajectories in early lymphocyte differentiation, we identified differentially expressed surface markers on lineage-negative lymphoid progenitors (LPs). Single-cell polymerase chain reaction experiments allowed us to link surface marker expression to that of lineage-associated transcription factors (TFs) and identify GFRA2 and BST1 as markers of early B cells. Functional analyses in vitro and in vivo as well as single-cell gene expression analyses supported that surface expression of these proteins defined distinct subpopulations that include cells from both the classical common LPs (CLPs) and Fraction A compartments. The formation of the GFRA2-expressing stages of development depended on the TF EBF1, critical both for the activation of stage-specific target genes and modulation of the epigenetic landscape. Our data show that consecutive expression of Ly6D, GFRA2, and BST1 defines a developmental trajectory linking the CLP to the CD19(+) progenitor compartment.
Subject: COMMON LYMPHOID PROGENITORS
T-CELL DEVELOPMENT
MOUSE BONE-MARROW
TRANSCRIPTION FACTOR EBF
LINEAGE COMMITMENT
FACTOR PAX5
EXPRESSION
DIFFERENTIATION
RECEPTOR
STAGE
3111 Biomedicine
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