Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development : The Plausible Role of miR-139-5p and miR-375

Show full item record



Permalink

http://hdl.handle.net/10138/298732

Citation

Rekker , K , Tasa , T , Saare , M , Samuel , K , Kadastik , U , Karro , H , Goette , M , Salumets , A & Peters , M 2018 , ' Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development : The Plausible Role of miR-139-5p and miR-375 ' , International Journal of Molecular Sciences , vol. 19 , no. 12 , 3789 . https://doi.org/10.3390/ijms19123789

Title: Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development : The Plausible Role of miR-139-5p and miR-375
Author: Rekker, Kadri; Tasa, Tonis; Saare, Merli; Samuel, Kulli; Kadastik, Ulle; Karro, Helle; Goette, Martin; Salumets, Andres; Peters, Maire
Contributor: University of Helsinki, Clinicum
Date: 2018-12
Language: eng
Number of pages: 11
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
URI: http://hdl.handle.net/10138/298732
Abstract: microRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. However, as both endometrium and endometriotic lesions consist of different cell types in various proportions, it is not clear which cells contribute to variability in miRNA levels and the overall knowledge about cell-type specific miRNA expression in ectopic cells is scarce. Therefore, we utilized fluorescence-activated cell sorting to isolate endometrial stromal cells from paired endometrial and endometrioma biopsies and combined it with high-throughput sequencing to determine miRNA alterations in endometriotic stroma. The analysis revealed 149 abnormally expressed miRNAs in endometriotic lesions, including extensive upregulation of miR-139-5p and downregulation of miR-375 compared to eutopic cells. miRNA transfection experiments in the endometrial stromal cell line ST-T1b showed that the overexpression of miR-139-5p resulted in the downregulation of homeobox A9 (HOXA9) and HOXA10 expression, whereas the endothelin 1 (EDN1) gene was regulated by miR-375. The results of this study provide further insights into the complex molecular mechanisms involved in endometriosis pathogenesis and demonstrate the necessity for cell-type-specific analysis of ectopic tissues to understand the interactions between different cell populations in disease onset and progression.
Subject: endometriosis
ectopic stroma
microRNA
small RNA sequencing
EDN1
HOXA10
miR-139-5p
miR-375
ESTROGEN-RECEPTOR-ALPHA
GENE-EXPRESSION
MICRORNA
ENDOTHELIN-1
PATHOGENESIS
BETA
1182 Biochemistry, cell and molecular biology
3111 Biomedicine
1184 Genetics, developmental biology, physiology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
ijms_19_03789.pdf 457.1Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record