Genetic Association and Altered Gene Expression of CYBB in Multiple Sclerosis Patients

Visa fullständig post



Permalänk

http://hdl.handle.net/10138/298805

Citation

Cardamone , G , Paraboschi , E M , Soldà , G , Duga , S , Saarela , J & Asselta , R 2018 , ' Genetic Association and Altered Gene Expression of CYBB in Multiple Sclerosis Patients ' , Biomedicines , vol. 6 , no. 4 , 117 . https://doi.org/10.3390/biomedicines6040117

Titel: Genetic Association and Altered Gene Expression of CYBB in Multiple Sclerosis Patients
Författare: Cardamone, Giulia; Paraboschi, Elvezia Maria; Soldà, Giulia; Duga, Stefano; Saarela, Janna; Asselta, Rosanna
Medarbetare: University of Helsinki, Janna Saarela / Principal Investigator
Datum: 2018-12-18
Språk: eng
Sidantal: 15
Tillhör serie: Biomedicines
ISSN: 2227-9059
Permanenta länken (URI): http://hdl.handle.net/10138/298805
Abstrakt: Multiple sclerosis (MS) is a chronic neurological disorder characterized by inflammation, demyelination, and axonal damage. Increased levels of reactive oxygen species (ROS), produced by macrophages and leading to oxidative stress, have been implicated as mediators of demyelination and axonal injury in both MS and experimental autoimmune encephalomyelitis, the murine model of the disease. On the other hand, reduced ROS levels can increase susceptibility to autoimmunity. In this work, we screened for association with MS 11 single nucleotide polymorphisms (SNPs) and two microsatellite markers in the five genes (NCF1, NCF2, NCF4, CYBA, and CYBB) of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX2) system, the enzymatic pathway producing ROS in the brain and neural tissues, in 347 Finnish patients with MS and 714 unaffected family members. This analysis showed suggestive association signals for NCF1 and CYBB (lowest p = 0.038 and p = 0.013, respectively). Functional relevance for disease predisposition was further supported for the CYBB gene, by microarray analysis in CD4+/− mononuclear cells of 21 individuals from five Finnish multiplex MS families, as well as by real-time RT-PCRs performed on RNA extracted from peripheral blood mononuclear cells of an Italian replication cohort of 21 MS cases and 21 controls. Our results showed a sex-specific differential expression of CYBB, suggesting that this gene, and more in general the NOX2 system, deserve to be further investigated for their possible role in MS.
Subject: 1184 Genetics, developmental biology, physiology
3111 Biomedicine
Licens:


Filer under denna titel

Totalt antal nerladdningar: Laddar...

Filer Storlek Format Granska
biomedicines_06_00117.pdf 998.9Kb PDF Granska/Öppna

Detta dokument registreras i samling:

Visa fullständig post