INNUENDO: A cross-sectoral platform for the integration of genomics in the surveillance of food-borne pathogens

Show full item record



Llarena , A-K , Ribeiro-Gonçalves , B F , Nuno Silva , D , Halkilahti , J , Machado , M P , Da Silva , M S , Jaakkonen , A , Isidro , J , Hämäläinen , C , Joenperä , J , Borges , V , Viera , L , Gomes , J P , Correia , C , Lunden , J , Laukkanen-Ninios , R , Fredriksson-Ahomaa , M , Bikandi , J , Millan , R S , Martinez-Ballesteros , I , Laorden , L , Mäesaar , M , Grantina-Ievina , L , Hilbert , F , Garaizar , J , Oleastro , M , Nevas , M , Salmenlinna , S , Hakkinen , M , Carriço , J A & Rossi , M 2018 , ' INNUENDO: A cross-sectoral platform for the integration of genomics in the surveillance of food-borne pathogens ' , EFSA Supporting Publications , vol. 15 , no. 11 , pp. 1498E .

Title: INNUENDO: A cross-sectoral platform for the integration of genomics in the surveillance of food-borne pathogens
Author: Llarena, Ann-Katrin; Ribeiro-Gonçalves, Bruno Filipe; Nuno Silva, Diogo; Halkilahti, Jani; Machado, Miguel Paulo; Da Silva, Mickael Santos; Jaakkonen, Anniina; Isidro, Joana; Hämäläinen, Crista; Joenperä, Jasmin; Borges, Vítor; Viera, Luìs; Gomes, João Paulo; Correia, Cristina; Lunden, Janne; Laukkanen-Ninios, Riikka; Fredriksson-Ahomaa, Maria; Bikandi, Joseba; Millan, Rosario San; Martinez-Ballesteros, Ilargi; Laorden, Lorena; Mäesaar, Mihael; Grantina-Ievina, Lelde; Hilbert, Friederike; Garaizar, Javier; Oleastro, Mónica; Nevas, Mari; Salmenlinna, Saara; Hakkinen, Marjaana; Carriço, João André; Rossi, Mirko
Contributor organization: Mirko Rossi Research Group
Food Hygiene and Environmental Health
Faculty of Veterinary Medicine
Janne Lundén / Principal Investigator
Maria Fredriksson-Ahomaa / Principal Investigator
Hannu Korkeala / Principal Investigator
Mari Nevas / Principal Investigator
Food Control research group
Date: 2018-11-26
Language: eng
Number of pages: 142
Belongs to series: EFSA Supporting Publications
ISSN: 2397-8325
Abstract: Abstract In response to the EFSA call New approaches in identifying and characterizing microbial and chemical hazards, the project INNUENDO ( aimed to design an analytical platform and standard procedures for the use of whole-genome sequencing in surveillance and outbreak investigation of food-borne pathogens. The project firstly attempted to identify existing flaws and needs, and then to provide applicable cross-sectorial solutions. The project focused in developing a platform for small countries with limited economical and personnel resources. To achieve these goals, we applied a user-centered design strategy involving the end-users, such as microbiologists in public health and veterinary authorities, in every step of the design, development and implementation phases. As a result, we delivered the INNUENDO Platform V1.0 (, a stand-alone, portable, open-source, end-to-end system for the management, analysis, and sharing of bacterial genomic data. The platform uses Nextflow workflow manager to assemble analytical software modules in species-specific protocols that can be run using a user-friendly interface. The reproducibility of the process is ensured by using Docker containers and throught the annotation of the whole process using an ontology. Several modules, available at, have been developed including: genome assembly and species confirmation; fast genome clustering; in silico typing; standardized species-specific phylogenetic frameworks for Campylobacter jejuni, Yersinia enterocolitica, Salmonella enterica and Escherichia coli based on an innovative gene-by-gene methodology; quality control measures from raw reads to allele calling; reporting system; a built-in communication protocols and a strain classification system enabling smooth communication during outbreak investigation. As proof-of-concepts, the proposed solutions have been thoroughly tested in simulated outbreak conditions by several public health and veterinary agencies across Europe. The results have been widely disseminated through several channels (web-sites, scientific publications, organization of workshops). The INNUENDO Platform V1.0 is effectively one of the models for the usage of open-source software in genomic epidemiology.
Subject: 416 Food Science
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
Llarena_et_al_2 ... upporting_Publications.pdf 5.200Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record