Toxin Variability Estimations of 68 Alexandrium ostenfeldii (Dinophyceae) Strains from The Netherlands Reveal a Novel Abundant Gymnodimine

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Martens , H , Tillmann , U , Harju , K , Dell'Aversano , C , Tartaglione , L & Krock , B 2017 , ' Toxin Variability Estimations of 68 Alexandrium ostenfeldii (Dinophyceae) Strains from The Netherlands Reveal a Novel Abundant Gymnodimine ' , Microorganisms , vol. 5 , no. 2 , UNSP 29 . https://doi.org/10.3390/microorganisms5020029

Title: Toxin Variability Estimations of 68 Alexandrium ostenfeldii (Dinophyceae) Strains from The Netherlands Reveal a Novel Abundant Gymnodimine
Author: Martens, Helge; Tillmann, Urban; Harju, Kirsi; Dell'Aversano, Carmela; Tartaglione, Luciana; Krock, Bernd
Contributor: University of Helsinki, VERIFIN
Date: 2017-06
Language: eng
Number of pages: 24
Belongs to series: Microorganisms
ISSN: 2076-2607
URI: http://hdl.handle.net/10138/299110
Abstract: Alexandrium ostenfeldii is a toxic dinoflagellate that has recently bloomed in Ouwerkerkse Kreek, The Netherlands, and which is able to cause a serious threat to shellfish consumers and aquacultures. We used a large set of 68 strains to the aim of fully characterizing the toxin profiles of the Dutch A. ostenfeldii in consideration of recent reports of novel toxins. Alexandrium ostenfeldii is known as a causative species of paralytic shellfish poisoning, and consistently in the Dutch population we determined the presence of several paralytic shellfish toxins (PST) including saxitoxin (STX), GTX2/3 (gonyautoxins), B1 and C1/C2. We also examined the production of spiroimine toxins by the Dutch A. ostenfeldii strains. An extensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed a high intraspecific variability of spirolides (SPX) and gymnodimines (GYM). Spirolides included 13-desMethyl-spirolide C generally as the major compound and several other mostly unknown SPX-like compounds that were detected and characterized. Besides spirolides, the presence of gymnodimine A and 12-Methyl-gymnodimine A was confirmed, together with two new gymnodimines. One of these was tentatively identified as an analogue of gymnodimine D and was the most abundant gymnodimine (calculated cell quota up to 274 pg cell(-1), expressed as GYM A equivalents). Our multi-clonal approach adds new analogues to the increasing number of compounds in these toxin classes and revealed a high strain variability in cell quota and in toxin profile of toxic compounds within a single population.
Subject: Alexandrium ostenfeldii
PSP-toxins
spiroimines
spirolides
gymnodimines
liquid chromatography-tandem mass spectrometry
SPIROLIDE MARINE TOXINS
MASS-SPECTROMETRY
PERUVIANUM BALECH
MENDIOLA BALECH
INTRASPECIFIC VARIABILITY
SHELLFISH TOXINS
NEW-ZEALAND
BALTIC SEA
DINOFLAGELLATE
TANGEN
116 Chemical sciences
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