In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography

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Kestilä , I , Thevenot , J , Finnilä , M A , Karhula , S S , Hadjab , I , Kauppinen , S , Garon , M , Quenneville , E , Haapea , M , Rieppo , L , Pritzker , K P , Buschmann , M D , Nieminen , H J & Saarakkala , S 2018 , ' In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography ' , Osteoarthritis and Cartilage , vol. 26 , no. 8 , pp. 1118-1126 . https://doi.org/10.1016/j.joca.2018.05.012

Title: In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography
Author: Kestilä, I.; Thevenot, J.; Finnilä, M. A.; Karhula, S. S.; Hadjab, I.; Kauppinen, S.; Garon, M.; Quenneville, E.; Haapea, M.; Rieppo, L.; Pritzker, K. P.; Buschmann, M. D.; Nieminen, H. J.; Saarakkala, S.
Other contributor: University of Helsinki, Department of Physics
Date: 2018-08
Language: eng
Number of pages: 9
Belongs to series: Osteoarthritis and Cartilage
ISSN: 1063-4584
DOI: https://doi.org/10.1016/j.joca.2018.05.012
URI: http://hdl.handle.net/10138/299172
Abstract: Objective: The aims of this study were: to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (mu CT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC. Method: The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop mCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semiautomatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1: 0-10%; zone 2: 10-40%; zone 3: 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6). Results: After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P Conclusion: We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/ chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA). (c) 2018 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Subject: Hexamethyldisilazane
Osteoarthritis
Segmentation
Morphology
3D analysis
SCANNING ELECTRON-MICROSCOPY
ADULT HUMAN KNEE
3-DIMENSIONAL MORPHOLOGY
CONFOCAL MICROSCOPY
PERICELLULAR MATRIX
CELL
OSTEOARTHRITIS
HEXAMETHYLDISILAZANE
MICROENVIRONMENT
ORGANIZATION
217 Medical engineering
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