Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera)

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dc.contributor.author Yang, Mei
dc.contributor.author Zhu, Lingping
dc.contributor.author Li, Ling
dc.contributor.author Li, Juanjuan
dc.contributor.author Xu, Liming
dc.contributor.author Feng, Ji
dc.contributor.author Liu, Yanling
dc.date.accessioned 2019-02-19T15:21:02Z
dc.date.available 2019-02-19T15:21:02Z
dc.date.issued 2017-01-31
dc.identifier.citation Yang , M , Zhu , L , Li , L , Li , J , Xu , L , Feng , J & Liu , Y 2017 , ' Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera) ' , Frontiers in plant science , vol. 8 , 80 . https://doi.org/10.3389/fpls.2017.00080
dc.identifier.other PURE: 82459386
dc.identifier.other PURE UUID: 44fb8727-19f3-49cc-b6ee-83e0361f0795
dc.identifier.other WOS: 000392864200001
dc.identifier.other Scopus: 85011964480
dc.identifier.uri http://hdl.handle.net/10138/299220
dc.description.abstract The predominant alkaloids in lotus leaves are aporphine alkaloids. These are the most important active components and have many pharmacological properties, but little is known about their biosynthesis. We used digital gene expression (DGE) technology to identify differentially-expressed genes (DEGs) between two lotus cultivars with different alkaloid contents at four leaf development stages. We also predicted potential genes involved in aporphine alkaloid biosynthesis by weighted gene co-expression network analysis (WGCNA). Approximately 335 billion nucleotides were generated; and 94% of which were aligned against the reference genome. Of 22 thousand expressed genes, 19,000 were differentially expressed between the two cultivars at the four stages. Gene Ontology (GO) enrichment analysis revealed that catalytic activity and oxidoreductase activity were enriched significantly in most pairwise comparisons. In Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, dozens of DEGs were assigned to the categories of biosynthesis of secondary metabolites, isoquinoline alkaloid biosynthesis, and flavonoid biosynthesis. The genes encoding norcoclaurine synthase (NCS), norcoclaurine 6-O-methyltransferase (6OMT), coclaurine N-methyltransferase (CNMT), N-methylcoclaurine 3'-hydroxylase (NMCH), and 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase (4'OMT) in the common pathways of benzylisoquinoline alkaloid biosynthesis and the ones encoding corytuberine synthase (CTS) in aporphine alkaloid biosynthetic pathway, which have been characterized in other plants, were identified in lotus. These genes had positive effects on alkaloid content, albeit with phenotypic lag. The WGCNA of DEGs revealed that one network module was associated with the dynamic change of alkaloid content. Eleven genes encoding proteins with methyltransferase, oxidoreductase and CYP450 activities were identified. These were surmised to be genes involved in aporphine alkaloid biosynthesis. This transcriptomic database provides new directions for future studies on clarifying the aporphine alkaloid pathway. en
dc.format.extent 19
dc.language.iso eng
dc.relation.ispartof Frontiers in plant science
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject lotus
dc.subject aporphine alkaloids
dc.subject co-expression network
dc.subject biosynthesis
dc.subject putative genes
dc.subject COEXPRESSION NETWORK ANALYSIS
dc.subject RNA-SEQ
dc.subject MOLECULAR-CLONING
dc.subject COPTIS-JAPONICA
dc.subject OPIUM POPPY
dc.subject BISBENZYLISOQUINOLINE ALKALOIDS
dc.subject BENZYLISOQUINOLINE ALKALOIDS
dc.subject HETEROLOGOUS EXPRESSION
dc.subject NORCOCLAURINE SYNTHASE
dc.subject SACRED LOTUS
dc.subject 1183 Plant biology, microbiology, virology
dc.title Digital Gene Expression Analysis Provides Insight into the Transcript Profile of the Genes Involved in Aporphine Alkaloid Biosynthesis in Lotus (Nelumbo nucifera) en
dc.type Article
dc.contributor.organization Department of Agricultural Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fpls.2017.00080
dc.relation.issn 1664-462X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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