Antioxidative CXXC Peptide Motif From Mesencephalic Astrocyte-Derived Neurotrophic Factor Antagonizes Programmed Cell Death

Show simple item record

dc.contributor University of Helsinki, Tallinn University of Technology en
dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor.author Božok, Valentina
dc.contributor.author Yu, Li-ying
dc.contributor.author Palgi, Jaan
dc.contributor.author Arumäe, Urmas
dc.date.accessioned 2019-02-22T15:19:01Z
dc.date.available 2019-02-22T15:19:01Z
dc.date.issued 2018-09-04
dc.identifier.citation Božok , V , Yu , L , Palgi , J & Arumäe , U 2018 , ' Antioxidative CXXC Peptide Motif From Mesencephalic Astrocyte-Derived Neurotrophic Factor Antagonizes Programmed Cell Death ' , Frontiers in Cell and Developmental Biology , vol. 6 , 106 . https://doi.org/10.3389/fcell.2018.00106 en
dc.identifier.issn 2296-634X
dc.identifier.other PURE: 122554413
dc.identifier.other PURE UUID: 621010cf-6c2f-4c37-94d4-353f6c9825cd
dc.identifier.other RIS: urn:FD69D6ACBA997A230EC9C37F101B0E30
dc.identifier.other WOS: 000455336200001
dc.identifier.other Scopus: 85053735632
dc.identifier.uri http://hdl.handle.net/10138/299380
dc.description.abstract Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a potent survival-promoting protein with neurorestorative effect for neurodegenerative diseases. Its mechanism of action, albeit poorly known, depends strongly on the CXXC motif (CKGC). Here we studied the survival-promoting properties of the CKGC tetrapeptide from MANF. In the Jurkat T lymphocytic cell line, CKGC potently inhibits death receptor Fas-induced apoptosis and mildly counteracts mitochondrial apoptosis and necroptosis. The peptide with serines instead of cysteines (SKGS) has no survival-promoting activity. The cytoprotective efficiency of the peptide against Fas-induced apoptosis is significantly improved by reduction of its cysteines by dithiotreitol, suggesting that it protects the cells via cysteine thiol groups, partially as an antioxidant. CKGC neutralizes the reactive oxygen species, maintains the mitochondrial membrane potential and prevents activation of the effector caspases in the Jurkat cells with activated Fas. The peptide does not require intracellular administration, as it is endocytosed and resides mainly in the Golgi. Finally, the peptide also potently promotes survival of cultured primary dopaminergic neurons. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Frontiers in Cell and Developmental Biology
dc.rights en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.subject 1184 Genetics, developmental biology, physiology en
dc.title Antioxidative CXXC Peptide Motif From Mesencephalic Astrocyte-Derived Neurotrophic Factor Antagonizes Programmed Cell Death en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.3389/fcell.2018.00106
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl
dc.contributor.pbl

Files in this item

Total number of downloads: Loading...

Files Size Format View
fcell_06_00106.pdf 2.849Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record