Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities

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Heuser , V D , Mansuri , N , Mogg , J , Kurki , S , Repo , H , Kronqvist , P , Carpen , O & Gardberg , M 2018 , ' Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities ' , Breast cancer-Basic and clinical research , vol. 12 , 1178223418792247 . https://doi.org/10.1177/1178223418792247

Title: Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities
Author: Heuser, Vanina D.; Mansuri, Naziha; Mogg, Jasper; Kurki, Samu; Repo, Heli; Kronqvist, Pauliina; Carpen, Olli; Gardberg, Maria
Contributor: University of Helsinki, Precision Cancer Pathology
Date: 2018-08-24
Language: eng
Number of pages: 12
Belongs to series: Breast cancer-Basic and clinical research
ISSN: 1178-2234
URI: http://hdl.handle.net/10138/299381
Abstract: Basal-like breast cancer is an aggressive form of breast cancer with limited treatment options. The subgroup can be identified immunohistochemically, by lack of hormone receptor expression combined with expression of basal markers such as CK5/6 and/or epidermal growth factor receptor (EGFR). In vitro, several regulators of the actin cytoskeleton are essential for efficient invasion of basal-like breast cancer cell lines. Whether these proteins are expressed in vivo determines the applicability of these findings in clinical settings. The actin-regulating formin protein FHOD1 participates in invasion of the triple-negative breast cancer cell line MDA-MB-231. Here, we measure the expression of FHOD1 protein in clinical triple-negative breast cancers by using immunohistochemistry and further characterize the expression of another formin protein, INF2. We report that basal-like breast cancers frequently overexpress formin proteins FHOD1 and INF2. In cell studies using basal-like breast cancer cell lines, we show that knockdown of FHOD1 or INF2 interferes with very similar processes: maintenance of cell shape, migration, invasion, and proliferation. Inhibition of EGFR, PI3K, or mitogen-activated protein kinase activity does not alter the expression of FHOD1 and INF2 in these cell lines. We conclude that the experimental studies on these formins have implications in the clinical behavior of basal-like breast cancer.
Subject: Formin
FHOD1
INF2
cytoskeleton
actin
triple-negative breast cancer
basal-like breast cancer
BT-549
MDA-MB-231
INTERNATIONAL EXPERT CONSENSUS
CLINICAL-IMPLICATIONS
GENE-EXPRESSION
PRIMARY THERAPY
ACTIN-FILAMENT
CELLS
HIGHLIGHTS
CARCINOMA
MIGRATION
DYNAMICS
3122 Cancers
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