Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities

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dc.contributor.author Heuser, Vanina D.
dc.contributor.author Mansuri, Naziha
dc.contributor.author Mogg, Jasper
dc.contributor.author Kurki, Samu
dc.contributor.author Repo, Heli
dc.contributor.author Kronqvist, Pauliina
dc.contributor.author Carpen, Olli
dc.contributor.author Gardberg, Maria
dc.date.accessioned 2019-02-22T15:24:01Z
dc.date.available 2019-02-22T15:24:01Z
dc.date.issued 2018-08-24
dc.identifier.citation Heuser , V D , Mansuri , N , Mogg , J , Kurki , S , Repo , H , Kronqvist , P , Carpen , O & Gardberg , M 2018 , ' Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities ' , Breast cancer-Basic and clinical research , vol. 12 , 1178223418792247 . https://doi.org/10.1177/1178223418792247
dc.identifier.other PURE: 122557530
dc.identifier.other PURE UUID: a1fda0be-0f3d-4d2f-b6c8-127cd062e4a8
dc.identifier.other WOS: 000445704800001
dc.identifier.other Scopus: 85055126948
dc.identifier.uri http://hdl.handle.net/10138/299381
dc.description.abstract Basal-like breast cancer is an aggressive form of breast cancer with limited treatment options. The subgroup can be identified immunohistochemically, by lack of hormone receptor expression combined with expression of basal markers such as CK5/6 and/or epidermal growth factor receptor (EGFR). In vitro, several regulators of the actin cytoskeleton are essential for efficient invasion of basal-like breast cancer cell lines. Whether these proteins are expressed in vivo determines the applicability of these findings in clinical settings. The actin-regulating formin protein FHOD1 participates in invasion of the triple-negative breast cancer cell line MDA-MB-231. Here, we measure the expression of FHOD1 protein in clinical triple-negative breast cancers by using immunohistochemistry and further characterize the expression of another formin protein, INF2. We report that basal-like breast cancers frequently overexpress formin proteins FHOD1 and INF2. In cell studies using basal-like breast cancer cell lines, we show that knockdown of FHOD1 or INF2 interferes with very similar processes: maintenance of cell shape, migration, invasion, and proliferation. Inhibition of EGFR, PI3K, or mitogen-activated protein kinase activity does not alter the expression of FHOD1 and INF2 in these cell lines. We conclude that the experimental studies on these formins have implications in the clinical behavior of basal-like breast cancer. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Breast cancer-Basic and clinical research
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Formin
dc.subject FHOD1
dc.subject INF2
dc.subject cytoskeleton
dc.subject actin
dc.subject triple-negative breast cancer
dc.subject basal-like breast cancer
dc.subject BT-549
dc.subject MDA-MB-231
dc.subject INTERNATIONAL EXPERT CONSENSUS
dc.subject CLINICAL-IMPLICATIONS
dc.subject GENE-EXPRESSION
dc.subject PRIMARY THERAPY
dc.subject ACTIN-FILAMENT
dc.subject CELLS
dc.subject HIGHLIGHTS
dc.subject CARCINOMA
dc.subject MIGRATION
dc.subject DYNAMICS
dc.subject 3122 Cancers
dc.title Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer : Association With Basal Markers and Functional Activities en
dc.type Article
dc.contributor.organization Precision Cancer Pathology
dc.contributor.organization Department of Pathology
dc.contributor.organization Medicum
dc.contributor.organization Clinicum
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1177/1178223418792247
dc.relation.issn 1178-2234
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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