Role of osteopontin and its regulation in pancreatic islet

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Cai , M , Bompada , P , Salehi , A , Acosta , J R , Prasad , R B , Atac , D , Laakso , M , Groop , L & De Marinis , Y 2018 , ' Role of osteopontin and its regulation in pancreatic islet ' , Biochemical and Biophysical Research Communications , vol. 495 , no. 1 , pp. 1426-1431 . https://doi.org/10.1016/j.bbrc.2017.11.147

Title: Role of osteopontin and its regulation in pancreatic islet
Author: Cai, Mengyin; Bompada, Pradeep; Salehi, Albert; Acosta, Juan R.; Prasad, Rashmi B.; Atac, David; Laakso, Markku; Groop, Leif; De Marinis, Yang
Contributor: University of Helsinki, Centre of Excellence in Complex Disease Genetics
Date: 2018-01-01
Language: eng
Number of pages: 6
Belongs to series: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
URI: http://hdl.handle.net/10138/299382
Abstract: Osteopontin (OPN) is involved in various physiological processes and also implicated in multiple pathological states. It has been suggested that OPN may have a role in type 2 diabetes (T2D) by protecting pancreatic islets and interaction with incretins. However, the regulation and function of OPN in islets, especially in humans, remains largely unexplored. In this study, we performed our investigations on both diabetic mouse model SUR1-E1506K+/+ and islets from human donors. We demonstrated that OPN protein, secretion and gene expression was elevated in the diabetic SUR1-E1506K+/+ islets. We also showed that high glucose and incretins simultaneously stimulated islet OPN secretion. In islets from human cadaver donors, OPN gene expression was elevated in diabetic islets, and externally added OPN significantly increased glucose-stimulated insulin secretion (GSIS) from diabetic but not normal glycemic donors. The increase in GSIS by OPN in diabetic human islets was Ca2+ dependent, which was abolished by Ca2+-channel inhibitor isradipine. Furthermore, we also confirmed that OPN promoted cell metabolic activity when challenged by high glucose. These observations provided evidence on the protective role of OPN in pancreatic islets under diabetic condition, and may point to novel therapeutic targets for islet protection in T2D. (C) 2017 Elsevier Inc. All rights reserved.
Subject: Osteopontin
Diabetes
Hyperglycemia
Islets
Insulin
Incretins
SMOOTH-MUSCLE-CELLS
POTENTIAL CLINICAL-APPLICATIONS
ADIPOSE-TISSUE
EPIGENETIC REGULATION
NITRIC-OXIDE
EXPRESSION
MUTATION
CALCIUM
OBESITY
KIDNEY
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
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