Aryl hydrocarbon receptor is indispensable for β-naphthoflavone-induced novel food avoidance and may be involved in LiCl-triggered conditioned taste aversion in rats

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Pohjanvirta , R & Mahiout , S 2019 , ' Aryl hydrocarbon receptor is indispensable for β-naphthoflavone-induced novel food avoidance and may be involved in LiCl-triggered conditioned taste aversion in rats ' , Physiology & Behavior , vol. 204 , pp. 58-64 . https://doi.org/10.1016/j.physbeh.2019.02.014

Title: Aryl hydrocarbon receptor is indispensable for β-naphthoflavone-induced novel food avoidance and may be involved in LiCl-triggered conditioned taste aversion in rats
Author: Pohjanvirta, Raimo; Mahiout, Selma
Contributor: University of Helsinki, Raimo Pohjanvirta / Principal Investigator
University of Helsinki, Raimo Pohjanvirta / Principal Investigator
Date: 2019-05-15
Language: eng
Number of pages: 7
Belongs to series: Physiology & Behavior
ISSN: 0031-9384
URI: http://hdl.handle.net/10138/299502
Abstract: Previous studies have shown that several aryl hydrocarbon receptor (AHR) agonists, including β-naphthoflavone (BNF), elicit avoidance of novel food items in rodents, with this behavioral response displaying a similar doseresponse to hepatic induction of CYP1A1. The avoidance has been found to bear substantial similarity to conditioned taste avoidance/aversion (CTA). The present study set out to confirm the indispensability of AHR in the avoidance response, to verify whether vagal afferent fibers are involved in it, and to see if AHR signaling might interfere with the effect of the classic trigger of CTA, LiCl. To this end, globally AHR deficient (AHRKO) or vagotomized wildtype rats were treated by gavage with 60 mg/kg BNF or ip with 0.15M LiCl (4 ml/kg), and presented with chocolate which was either novel or familiar to them. Both the avoidance response and Cyp1a1 induction were missing in AHRKO rats. In contrast, Ahr+/− rats exhibited them in full, save for a single outlier. Total subdiaphragmatic vagotomy failed to interfere with the avoidance of novel or familiar chocolate or induction of Cyp1a1. After LiCl administration, male AHRKO rats showed a significantly mitigated suppression of chocolate consumption compared with wildtype animals (~60% vs. ~10% of control chocolate intake, respectively). A similar tendency was seen in females, but they were less responsive to LiCl. These findings corroborate AHR as a prerequisite of the BNF-induced novel food avoidance, prove vagal afferents unlikely mediators of this response, and imply an unforeseen involvement of AHR signaling in the thoroughly-characterized CTA instigated by LiCl.
Subject: 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
ACQUISITION
AH RECEPTOR
AHR knockout rats
Aryl hydrocarbon receptor
BRAIN
Conditioned taste aversion
INDUCTION
LiCl
MICE
NEURONS
Novel food avoidance
PARABRACHIAL NUCLEUS
SUBDIAPHRAGMATIC VAGOTOMY
TOXICITY
Total subdiaphragmatic vagotomy
Vagotomized rats
beta-Naphthoflavone
416 Food Science
413 Veterinary science
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