Population genetics and characterization of Campylobacter jejuni isolates from western jackdaws and game birds in Finland

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Kovanen , S , Rossi , M , Pohja-Mykrä , M , Nieminen , T , Raunio-Saarnisto , M , Sauvala , M , Fredriksson-Ahomaa , M , Hänninen , M-L & Kivistö , R I 2019 , ' Population genetics and characterization of Campylobacter jejuni isolates from western jackdaws and game birds in Finland ' , Applied and Environmental Microbiology , vol. 85 , no. 4 , UNSP e02365-18 . https://doi.org/10.1128/AEM.02365-18

Title: Population genetics and characterization of Campylobacter jejuni isolates from western jackdaws and game birds in Finland
Author: Kovanen, Sara; Rossi, Mirko; Pohja-Mykrä, Mari; Nieminen, Timo; Raunio-Saarnisto, Mirja; Sauvala, Mikaela; Fredriksson-Ahomaa, Maria; Hänninen, Marja-Liisa; Kivistö, Rauni Inari
Contributor: University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Mirko Rossi Research Group
University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
University of Helsinki, Ruralia Institute, Seinäjoki
University of Helsinki, Food Hygiene and Environmental Health
University of Helsinki, Maria Fredriksson-Ahomaa / Principal Investigator
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Food Hygiene and Environmental Health
Date: 2019-02
Language: eng
Number of pages: 16
Belongs to series: Applied and Environmental Microbiology
ISSN: 0099-2240
URI: http://hdl.handle.net/10138/299505
Abstract: Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and pheasants (n = 9, 9%). Most of the western jackdaw and mallard duck C. jejuni isolates represented multilocus sequence typing (MLST) sequence types (STs) that diverged from those previously isolated from human patients and various animal species, whereas all pheasant isolates represented ST-19, a common ST among human patients and other hosts worldwide. Whole-genome MLST revealed that mallard duck ST-2314 and pheasant ST-19 isolates represented bacterial clones that were genetically highly similar to human isolates detected previously. Further analyses revealed that in addition to a divergent ClonalFrame genealogy, certain genomic characteristics of the western jackdaw C. jejuni isolates, e.g., a novel cdtABC gene cluster and the type VI secretion system (T6SS), may affect their host specificity and virulence. Game birds may thus pose a risk for acquiring campylobacteriosis; therefore, hygienic measures during slaughter and meat handling warrant special attention. IMPORTANCE The roles of environmental reservoirs, including wild birds, in the molecular epidemiology of Campylobacter jejuni have not been assessed in depth. Our results showed that game birds may pose a risk for acquiring campylobacteriosis, because they had C. jejuni genomotypes highly similar to human isolates detected previously. Therefore, hygienic measures during slaughter and meat handling warrant special attention. On the contrary, a unique phylogeny was revealed for the western jackdaw isolates, and certain genomic characteristics identified among these isolates are hypothesized to affect their host specificity and virulence. Comparative genomics within sequence types (STs), using whole-genome multilocus sequence typing (wgMLST), and phylogenomics are efficient methods to analyze the genomic relationships of C. jejuni isolates.
Subject: 1183 Plant biology, microbiology, virology
Campylobacter jejuni
antimicrobial resistance
comparative genomics
cytolethal distending toxin
mallard duck
pheasant
public health
western jackdaw
whole-genome sequencing
416 Food Science
3142 Public health care science, environmental and occupational health
Campylobacter jejuni
antimicrobial resistance
comparative genomics
cytolethal distending toxin
mallard duck
pheasant
public health
western jackdaw
whole-genome sequencing
CYTOLETHAL DISTENDING TOXIN
MULTILOCUS SEQUENCE TYPES
ANTIMICROBIAL RESISTANCE
ANNOTATION
VIRULENCE
HOST
IDENTIFICATION
COLONIZATION
TRANSMISSION
INFECTIONS
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