Establishment of a spontaneously transformed cell line (JU-PI) from a myxoinflammatory fibroblastic sarcoma

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Fagerstedt , K W , Salonen , T , Zhao , F , Kytölä , S , Böhling , T & Andersson , L C 2018 , ' Establishment of a spontaneously transformed cell line (JU-PI) from a myxoinflammatory fibroblastic sarcoma ' , Tumor Biology , vol. 40 , no. 5 . https://doi.org/10.1177/1010428318777936

Title: Establishment of a spontaneously transformed cell line (JU-PI) from a myxoinflammatory fibroblastic sarcoma
Author: Fagerstedt, K.W.; Salonen, T.; Zhao, F.; Kytölä, S.; Böhling, T.; Andersson, L.C.
Other contributor: University of Helsinki, Department of Pathology
University of Helsinki, Clinicum
University of Helsinki, Department of Pathology
University of Helsinki, Medicum


Date: 2018
Language: eng
Belongs to series: Tumor Biology
ISSN: 1010-4283
DOI: https://doi.org/10.1177/1010428318777936
URI: http://hdl.handle.net/10138/299676
Abstract: Myxoinflammatory fibroblastic sarcoma is a soft-tissue neoplasm most frequently found in the distal extremities of middle-aged adults. Most myxoinflammatory fibroblastic sarcoma are low-grade tumors with propensity for local recurrence after incomplete removal. We report a myxoinflammatory fibroblastic sarcoma which developed in the foot of a 41-year-old male and showed an exceptionally aggressive course with metastatic spread and fatal outcome within 16 months. We managed to establish a spontaneously transformed continuous cell line, called JU-PI, from a metastatic lesion. The JU-PI cells have a sub-tetraploid karyotype including the 1;10 chromosomal translocation and amplification of the proximal end of 3p; these features are considered genetic signatures of myxoinflammatory fibroblastic sarcoma. Both the primary tumor and the JU-PI cells showed nuclear expression of the TFE3 transcription factor but TFE3-activating chromosomal rearrangements were not found. To our knowledge, JU-PI is the first established myxoinflammatory fibroblastic sarcoma cell line. JU-PI cells offer a tool for investigating the molecular oncology of myxoinflammatory fibroblastic sarcoma. © 2018, © The Author(s) 2018.
Subject: dacarbazine
doxorubicin
etoposide
ifosfamide
transcription factor
transcription factor TFE3
unclassified drug
vincristine
antineoplastic agent
mesna
vincristine, abdominal tumor
adult
Article
cancer palliative therapy
cancer radiotherapy
case report
cell growth
chromosome 3
chromosome rearrangement
chromosome translocation
clinical article
clinical feature
comparative genomic hybridization
disease course
disease exacerbation
DNA isolation
fibrosarcoma
fibrosarcoma cell line
foot injury
gene activation
gene expression
human
human cell
human tissue
immunohistochemistry
JU-PI cell line
leg amputation
lung metastasis
male
microsatellite marker
myxoinflammatory fibroblastic sarcoma
patient history of surgery
polymerase chain reaction
priority journal
recurrent disease
retroperitoneal cancer
scar tissue
transmission electron microscopy
treatment response
tumor growth
whole body CT
cell proliferation
gene translocation
genetics
karyotype
mortality
pathology
primary cell culture
procedures
soft tissue tumor
transformed cell line
tumor recurrence, Adult
Antineoplastic Combined Chemotherapy Protocols
Cell Line, Transformed
Cell Proliferation
Dacarbazine
Doxorubicin
Fibrosarcoma
Humans
Ifosfamide
Karyotype
Male
Mesna
Neoplasm Recurrence, Local
Primary Cell Culture
Soft Tissue Neoplasms
Translocation, Genetic
Vincristine
3122 Cancers
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