Circulating pathogen-specific plasmablasts in female patients with upper genital tract infection

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http://hdl.handle.net/10138/299817

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Palkola , N V , Pakkanen , S H , Heikinheimo , O , Kantele , J M & Kantele , A 2018 , ' Circulating pathogen-specific plasmablasts in female patients with upper genital tract infection ' , Journal of Reproductive Immunology , vol. 126 , pp. 69-75 . https://doi.org/10.1016/j.jri.2018.02.005

Title: Circulating pathogen-specific plasmablasts in female patients with upper genital tract infection
Author: Palkola, Nina V.; Pakkanen, Sari H.; Heikinheimo, Oskari; Kantele, Jussi M.; Kantele, Anu
Contributor: University of Helsinki, Anu Kantele-Häkkinen Research Group
University of Helsinki, Clinicum
University of Helsinki, Clinicum
Date: 2018-04
Language: eng
Number of pages: 7
Belongs to series: Journal of Reproductive Immunology
ISSN: 0165-0378
URI: http://hdl.handle.net/10138/299817
Abstract: Mucosal antibodies constitute the first line of adaptive immune defence against invaders in the female genital tract (FGT), yet the sequence of events leading to their production is surprisingly poorly characterized. We explored the induction of pathogen-specific antibody-secreting cells (ASC) as a response to an acute infection in the upper FGT. We recruited 12 patients undergoing surgery due to an upper FGT infection (7/12 blood culture positive, 5/ 12 negative) and six healthy controls. Pathogens were sampled during surgery and PBMC collected in the acute phase of the disease (days 7-10). We searched by ELISPOT circulating pathogen-specific ASC and explored their frequency, immunoglobulin isotype distribution, and expressions of homing receptors (alpha(4)beta(7), L-selectin, and CLA). All patients had circulating ASC specific to the infective bacteria; the geometric mean was 434 (95%CI 155-1234) ASC (IgA + IgG + IgM)/10(6) PBMC. IgA ASC predominated in 7/12, IgG ASC in 3/12, and IgM ASC in 2/12 cases. Of all the pathogen-specific ASC, 60% expressed alpha(4)beta(7), 67% L-selectin, and 9% CLA. This study is the first to show induction of pathogen-specific ASC in the peripheral blood in bacterial infection in the human FGT. Our findings reveal that such FGT-originating pathogen-specific ASC are predominated by IgA ASC and exhibit a homing receptor profile resembling that of ASC in acute urinary tract infection. The data thus suggest a characteristic profile shared by the urogenital tract.
Subject: 3123 Gynaecology and paediatrics
Lymphocyte homing
adhesion molecule
homing receptor
pathogen-specific antibody-secreting cell
plasmablast
upper female genital tract
ELISPOT
CUTANEOUS LYMPHOCYTE ANTIGEN
HUMAN B-CELLS
SALMONELLA-TYPHI
PROTECTIVE IMMUNITY
CHLAMYDIA-TRACHOMATIS INFECTION
REPRODUCTIVE-TRACT
ANTIBODY-SECRETING CELLS
MUCOSAL IMMUNE-SYSTEM
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