Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6

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Lyytinen , O L , Starkova , D & Poranen , M M 2019 , ' Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6 ' , Microbial Cell Factories , vol. 18 , no. 29 , 29 . https://doi.org/10.1186/s12934-019-1079-z

Title: Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6
Author: Lyytinen, Outi Leena; Starkova, Daria; Poranen, Minna Marjetta
Contributor organization: Molecular and Integrative Biosciences Research Programme
Molecular and Translational Virology
Date: 2019-02-07
Language: eng
Number of pages: 9
Belongs to series: Microbial Cell Factories
ISSN: 1475-2859
DOI: https://doi.org/10.1186/s12934-019-1079-z
URI: http://hdl.handle.net/10138/299903
Abstract: BackgroundCystoviruses have a phospholipid envelope around their nucleocapsid. Such a feature is unique among bacterial viruses (i.e., bacteriophages) and the mechanisms of virion envelopment within a bacterial host are largely unknown. The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. The phi6 major envelope protein P9 and the non-structural protein P12 are essential for the envelopment of its virions. Co-expression of P9 and P12 in a Pseudomonas host results in the formation of intracellular vesicles that are potential intermediates in the phi6 virion assembly pathway. This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli.ResultsUsing transmission electron microscopy, we detected membranous structures in the cytoplasm of E. coli cells expressing P9. The density of the P9-specific membrane fraction was lower (approximately 1.13g/cm(3) in sucrose) than the densities of the bacterial cytoplasmic and outer membrane fractions. A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. Production of the GFP-tagged P9 vesicles required P12, which protected the fusion protein against proteolytic cleavage. Isolated vesicles contained predominantly P9-GFP, suggesting selective incorporation of P9-tagged fusion proteins into the vesicles.ConclusionsOur results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. Intracellular membrane structures are rare in bacteria, thus making them ideal chasses for cell-based vesicle production. The possibility to locate heterologous proteins into the P9-lipid vesicles facilitates the production of vesicular structures with novel properties. Such products have potential use in biotechnology and biomedicine.
Subject: DNA
Escherichia coli
Major envelope protein P9
Non-structural protein P12
Pseudomonas phage phi6
Vesicle production
Viral envelope formation
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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