Deciphering the Antibacterial Mode of Action of Alpha-Mangostin on Staphylococcus epidermidis RP62A Through an Integrated Transcriptomic and Proteomic Approach

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dc.contributor.author Sivaranjani, Murugesan
dc.contributor.author Leskinen, Katarzyna
dc.contributor.author Aravindraja, Chairmandurai
dc.contributor.author Saavalainen, Päivi
dc.contributor.author Pandian, Shunmugiah Karutha
dc.contributor.author Skurnik, Mikael
dc.contributor.author Ravi, Arumugam Veera
dc.date.accessioned 2019-03-11T08:58:02Z
dc.date.available 2019-03-11T08:58:02Z
dc.date.issued 2019-02-06
dc.identifier.citation Sivaranjani , M , Leskinen , K , Aravindraja , C , Saavalainen , P , Pandian , S K , Skurnik , M & Ravi , A V 2019 , ' Deciphering the Antibacterial Mode of Action of Alpha-Mangostin on Staphylococcus epidermidis RP62A Through an Integrated Transcriptomic and Proteomic Approach ' , Frontiers in Microbiology , vol. 10 , 150 . https://doi.org/10.3389/fmicb.2019.00150
dc.identifier.other PURE: 123134057
dc.identifier.other PURE UUID: c140a962-2e98-4a4c-bc12-d759ac85ba19
dc.identifier.other WOS: 000458045900001
dc.identifier.other ORCID: /0000-0001-8791-9260/work/55350496
dc.identifier.uri http://hdl.handle.net/10138/300026
dc.description.abstract Background: Alpha-mangostin (alpha-MG) is a natural xanthone reported to exhibit rapid bactericidal activity against Gram-positive bacteria, and may therefore have potential clinical application in healthcare sectors. This study sought to identify the impact of alpha-MG on Staphylococcus epidermidis RP62A through integrated advanced omic technologies. Methods: S. epidermidis was challenged with sub-MIC (0.875 mu g/ml) of alpha-MG at various time points and the differential expression pattern of genes/proteins were analyzed in the absence and presence of alpha-MG using RNA sequencing and LC-MS/MS experiments. Bioinformatic tools were used to categorize the biological processes, molecular functions and KEGG pathways of differentially expressed genes/proteins. qRT-PCR was employed to validate the results obtained from these analyses. Results: Transcriptomic and proteomic profiling of alpha-MG treated cells indicated that genes/proteins affected by alpha-MG treatment were associated with diverse cellular functions. The greatest reduction in expression was observed in transcription of genes conferring cytoplasmic membrane integrity (yidC2, secA and mscL), cell division (ftsY and divlB), teichoic acid biosynthesis (tagG and dltA), fatty-acid biosynthesis (accB, accC, fabD, fabH, fabl, and fabZ), biofilm formation (icaA) and DNA replication and repair machinery (polA, polC, dnaE, and uvrA). Those with increased expression were involved in oxidative (katA and sodA) and cellular stress response (clpB, clpC, groEL, and asp23). The qRT-PCR analysis substantiated the results obtained from transcriptomic and proteomic profiling studies. Conclusion: Combining transcriptomic and proteomic methods provided comprehensive information about the antibacterial mode of action of alpha-MG. The obtained results suggest that alpha-MG targets S. epidermidis through multifarious mechanisms, and especially prompts that loss of cytoplasmic membrane integrity leads to rapid onset of bactericidal activity. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof Frontiers in Microbiology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject RNA-sequencing
dc.subject LC-MS/MS
dc.subject bactericidal
dc.subject alpha-mangostin
dc.subject cytoplasmic membrane
dc.subject XANTHONE DERIVATIVES
dc.subject BACILLUS-SUBTILIS
dc.subject ANTIMICROBIALS
dc.subject DISCOVERY
dc.subject PLANT
dc.subject RESISTANCE
dc.subject VANCOMYCIN
dc.subject INHIBITOR
dc.subject RELEVANCE
dc.subject BIOFILMS
dc.subject 3111 Biomedicine
dc.subject 1183 Plant biology, microbiology, virology
dc.title Deciphering the Antibacterial Mode of Action of Alpha-Mangostin on Staphylococcus epidermidis RP62A Through an Integrated Transcriptomic and Proteomic Approach en
dc.type Article
dc.contributor.organization Immunobiology Research Program
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Medicum
dc.contributor.organization Research Programs Unit
dc.contributor.organization University of Helsinki
dc.contributor.organization Immunomics
dc.contributor.organization Mikael Skurnik / Principal Investigator
dc.contributor.organization Department of Diagnostics and Therapeutics
dc.contributor.organization Clinicum
dc.contributor.organization HUSLAB
dc.contributor.organization Helsinki One Health (HOH)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fmicb.2019.00150
dc.relation.issn 1664-302X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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