Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip electrophoresis-electrochemical detection

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Ollikainen , E , Aitta-aho , T , Koburg , M , Kostiainen , R & Sikanen , T 2019 , ' Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip electrophoresis-electrochemical detection ' , Scientific Reports , vol. 9 , 3311 . https://doi.org/10.1038/s41598-019-40116-5

Title: Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip electrophoresis-electrochemical detection
Author: Ollikainen, Elisa; Aitta-aho, Teemu; Koburg, Michaela; Kostiainen, Risto; Sikanen, Tiina
Contributor organization: Division of Pharmaceutical Chemistry and Technology
Drug Research Program
Department of Pharmacology
Medicum
Faculty of Medicine
Risto Kostiainen / Principal Investigator
Tiina Sikanen / Chemical Microsystems Lab
Date: 2019-03
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-019-40116-5
URI: http://hdl.handle.net/10138/300096
Abstract: Animal studies remain an essential part of drug discovery since in vitro models are not capable of describing the complete living organism. We developed and qualified a microchip electrophoresis-electrochemical detection (MCE-EC) method for rapid analysis of morphine in mouse plasma using a commercial MCE-EC device. Following liquid-liquid extraction (LLE), we achieved within-run precision of 3.7 and 4.5% (coefficient of variation, CV, n = 6) and accuracy of 106.9% and 100.7% at biologically relevant morphine concentrations of 5 and 20 mu M in plasma, respectively. The same method was further challenged by morphine detection in mouse brain homogenates with equally good within-run precision (7.8% CV, n = 5) at 1 mu M concentration. The qualified method was applied to analyze a set of plasma and brain homogenate samples derived from a behavioral animal study. After intraperitoneal administration of 20 mg/kg morphine hydrochloride, the detected morphine concentrations in plasma were between 6.7 and 17 mu M. As expected, the morphine concentrations in the brain were significantly lower, ca. 80125 nM (280-410 pg morphine/mg dissected brain), and could only be detected after preconcentration achieved during LLE. In all, the microchip-based separation system is proven feasible for rapid analysis of morphine to provide supplementary chemical information to behavioral animal studies.
Subject: PERFORMANCE LIQUID-CHROMATOGRAPHY
AMPEROMETRIC DETECTION
SEPARATION
INSTRUMENT
ELECTRODE
DEVICE
DRUGS
ASSAY
PAIN
317 Pharmacy
116 Chemical sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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