Ollikainen , E , Aitta-aho , T , Koburg , M , Kostiainen , R & Sikanen , T 2019 , ' Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip electrophoresis-electrochemical detection ' , Scientific Reports , vol. 9 , 3311 . https://doi.org/10.1038/s41598-019-40116-5
Title: | Rapid analysis of intraperitoneally administered morphine in mouse plasma and brain by microchip electrophoresis-electrochemical detection |
Author: | Ollikainen, Elisa; Aitta-aho, Teemu; Koburg, Michaela; Kostiainen, Risto; Sikanen, Tiina |
Contributor organization: | Division of Pharmaceutical Chemistry and Technology Drug Research Program Department of Pharmacology Medicum Faculty of Medicine Risto Kostiainen / Principal Investigator Tiina Sikanen / Chemical Microsystems Lab |
Date: | 2019-03 |
Language: | eng |
Number of pages: | 9 |
Belongs to series: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | https://doi.org/10.1038/s41598-019-40116-5 |
URI: | http://hdl.handle.net/10138/300096 |
Abstract: | Animal studies remain an essential part of drug discovery since in vitro models are not capable of describing the complete living organism. We developed and qualified a microchip electrophoresis-electrochemical detection (MCE-EC) method for rapid analysis of morphine in mouse plasma using a commercial MCE-EC device. Following liquid-liquid extraction (LLE), we achieved within-run precision of 3.7 and 4.5% (coefficient of variation, CV, n = 6) and accuracy of 106.9% and 100.7% at biologically relevant morphine concentrations of 5 and 20 mu M in plasma, respectively. The same method was further challenged by morphine detection in mouse brain homogenates with equally good within-run precision (7.8% CV, n = 5) at 1 mu M concentration. The qualified method was applied to analyze a set of plasma and brain homogenate samples derived from a behavioral animal study. After intraperitoneal administration of 20 mg/kg morphine hydrochloride, the detected morphine concentrations in plasma were between 6.7 and 17 mu M. As expected, the morphine concentrations in the brain were significantly lower, ca. 80125 nM (280-410 pg morphine/mg dissected brain), and could only be detected after preconcentration achieved during LLE. In all, the microchip-based separation system is proven feasible for rapid analysis of morphine to provide supplementary chemical information to behavioral animal studies. |
Subject: |
PERFORMANCE LIQUID-CHROMATOGRAPHY
AMPEROMETRIC DETECTION SEPARATION INSTRUMENT ELECTRODE DEVICE DRUGS ASSAY PAIN 317 Pharmacy 116 Chemical sciences |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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