Sequencing the plastid genome of giant ragweed (Ambrosia trifida, Asteraceae) from a herbarium specimen

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Sablok , G , Amiryousefi , A , He , X , Hyvönen , J T & Poczai , P 2019 , ' Sequencing the plastid genome of giant ragweed (Ambrosia trifida, Asteraceae) from a herbarium specimen ' , Frontiers in plant science , vol. 10 , 218 . https://doi.org/10.3389/fpls.2019.00218

Title: Sequencing the plastid genome of giant ragweed (Ambrosia trifida, Asteraceae) from a herbarium specimen
Author: Sablok, Gaurav; Amiryousefi, Ali; He, Xiaolan; Hyvönen, Jaakko Tapani; Poczai, Péter
Contributor organization: Biosciences
Finnish Museum of Natural History
Organismal and Evolutionary Biology Research Programme
Viikki Plant Science Centre (ViPS)
Embryophylo
Plant Biology
Date: 2019-02-28
Language: eng
Number of pages: 18
Belongs to series: Frontiers in plant science
ISSN: 1664-462X
DOI: https://doi.org/10.3389/fpls.2019.00218
URI: http://hdl.handle.net/10138/300101
Abstract: We report the first plastome sequence of giant ragweed (Ambrosia trifida); with this new genome information, we assessed the phylogeny of Asteraceae and the transcriptional profiling against glyphosate resistance in giant ragweed. Assembly and genic features show a normal angiosperm quadripartite plastome structure with no signatures of deviation in gene directionality. Comparative analysis revealed large inversions across the plastome of giant ragweed and the previously sequenced members of the plant family. Asteraceae plastid genomes contain two inversions of 22.8 and 3.3 kb; the former is located between trnS-GCU and trnG-UCC genes, and the latter between trnE-UUC and trnT-GGU genes. The plastid genome sequences of A. trifida and the related species, Ambrosia artemisiifolia, are identical in gene content and arrangement, but they differ in length. The phylogeny is well-resolved and congruent with previous hypotheses about the phylogenetic relationship of Asteraceae. Transcriptomic analysis revealed divergence in the relative expressions at the exonic and intronic levels, providing hints toward the ecological adaptation of the genus. Giant ragweed shows various levels of glyphosate resistance, with introns displaying higher expression patterns at resistant time points after the assumed herbicide treatment.
Subject: ARTEMISIIFOLIA L.
Ambrosia trifida
COMMON RAGWEED
COMPLETE CHLOROPLAST GENOME
EARLY EVOLUTION
GENETIC DIVERSITY
GLYPHOSATE RESISTANCE
HERBICIDE RESISTANCE
INVERSION VARIATION
NATIVE RANGE
RECURRENT SELECTION
chloroplast genome
genome skimming
glyphosate
herbicide resistance
invasive plants
noxious weeds
ragweed
1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion
Funder: SUOMEN AKATEMIA
LUOMUS
Grant number:


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