Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish

Show full item record



Permalink

http://hdl.handle.net/10138/300208

Citation

Sahu , M P , Pazos-Boubeta , Y , Pajanoja , C , Rozov , S , Panula , P & Castren , E 2019 , ' Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish ' , Scientific Reports , vol. 9 , 2036 . https://doi.org/10.1038/s41598-019-39347-3

Title: Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish
Author: Sahu, Madhusmita Priyadarshini; Pazos-Boubeta, Yago; Pajanoja, Ceren; Rozov, Stanislav; Panula, Pertti; Castren, Eero
Contributor organization: Neuroscience Center
University of Helsinki
Helsinki Institute of Life Science HiLIFE
Laboratory of Neurotherapeutics
Helsinki In Vivo Animal Imaging Platform (HAIP)
Department of Anatomy
Pertti Panula / Principal Investigator
Medicum
Eero Castren / Principal Investigator
Date: 2019-02-14
Language: eng
Number of pages: 13
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-019-39347-3
URI: http://hdl.handle.net/10138/300208
Abstract: Neurotrophins and their receptors have highly conserved evolutionary lineage in vertebrates including zebrafish. The NTRK2 receptor has two isoforms in zebrafish, Ntrk2a and Ntrk2b. The spatio-temporal expression pattern of bdnf and ntrk2b in the zebrafish brain was studied using in situ hybridization. The robust and corresponding expression pattern of ntrk2b to bdnf suggests that ntrk2b is the key receptor for bdnf in the zebrafish brain, unlike its duplicate isoform ntrk2a. To study ntrk2b function, two different genetic strategies, the TILLING mutant and morpholino oligonucleotides (MO), were used. Specific subsets of the dopaminergic and serotonergic neuronal populations were affected in the mutants and morphants. The mutant showed anxiety-like behavior both in larval and adult stages. Our results consistently indicate that BDNF/NTRK2 signaling has a significant role in the development and maintenance of aminergic neuronal populations. Therefore, the ntrk2b-deficient zebrafish is well suited to study mechanisms relevant for psychiatric disorders attributed to a dysfunctional monoaminergic system.
Subject: ADULT ZEBRAFISH
BRAIN
BDNF
TRKB
EXPRESSION
SYSTEM
ACTIVATION
LOCALIZATION
PLASTICITY
PHENOTYPE
3112 Neurosciences
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


Files in this item

Total number of downloads: Loading...

Files Size Format View
s41598_019_39347_3.pdf 2.795Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record