Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut

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Banić , M , Uroić , K , Leboš Pavunc , A , Novak , J , Zorić , K , Durgo , K , Petković , H , Jamnik , P , Kazazić , S , Kazazić , S , Radović , S , Scalabrin , S , Hynönen , U , Šušković , J & Kos , B 2018 , ' Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut ' , LWT-Food Science and Technology , vol. 93 , pp. 257-267 . https://doi.org/10.1016/j.lwt.2018.03.054

Title: Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut
Author: Banić, Martina; Uroić, Ksenija; Leboš Pavunc, Andreja; Novak, Jasna; Zorić, Katarina; Durgo, Ksenija; Petković, Hrvoje; Jamnik, Polona; Kazazić, Saša; Kazazić, Snježana; Radović, Slobodanka; Scalabrin, Simone; Hynönen, Ulla; Šušković, Jagoda; Kos, Blaženka
Contributor: University of Helsinki, Veterinary Biosciences
Date: 2018-07
Language: eng
Number of pages: 11
Belongs to series: LWT-Food Science and Technology
ISSN: 0023-6438
URI: http://hdl.handle.net/10138/300244
Abstract: Abstract S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal (GI) conditions and during freeze-drying. Their survival in stress conditions was additionally enhanced by microencapsulation, especially when using alginate with gelatine as a matrix. Thus prepared cells were subjected to simulated GI conditions and their mortality was only 0.28 ± 0.45 log CFU/mL. Furthermore, a correlation between the high surface hydrophobicity and the remarkable aggregative capacity of SF9B strain was established. The results indicate a prominent role of Slps in adhesion to mucin, extracellular matrix (ECM) proteins, and particularly to Caco-2 cells, where the removal of Slps utterly abolished the adhesiveness of SF9B cells for 7.78 ± 0.25 log CFU/mL.
Subject: Lactobacillus brevis
Probiotics
Slps
Adhesion
Microencapsulation
STRAINS
ACIDOPHILUS M92
PATHOGEN
ADHESION PROPERTIES
BINDING-PROTEIN
ADHERENCE
MICROBIAL-CELLS
LACTIC-ACID BACTERIA
PLANTARUM
AGGREGATION
416 Food Science
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