Newly detected DNA viruses in juvenile nasopharyngeal angiofibroma (JNA) and oral and oropharyngeal squamous cell carcinoma (OSCC/OPSCC)

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Dickinson , A , Xu , M , Silen , S , Wang , Y , Fu , Y , Sadeghi , M , Toppinen , M , Carpen , T , Hedman , K , Mäkitie , A & Söderlund-Venermo , M 2019 , ' Newly detected DNA viruses in juvenile nasopharyngeal angiofibroma (JNA) and oral and oropharyngeal squamous cell carcinoma (OSCC/OPSCC) ' , European Archives of Oto-Rhino-Laryngology , vol. 276 , no. 2 , pp. 613-617 . https://doi.org/10.1007/s00405-018-5250-7

Title: Newly detected DNA viruses in juvenile nasopharyngeal angiofibroma (JNA) and oral and oropharyngeal squamous cell carcinoma (OSCC/OPSCC)
Author: Dickinson, Amy; Xu, Man; Silen, Suvi; Wang, Yilin; Fu, Yu; Sadeghi, Mohammadreza; Toppinen, Mari; Carpen, Timo; Hedman, Klaus; Mäkitie, Antti; Söderlund-Venermo, Maria
Other contributor: University of Helsinki, Clinicum
University of Helsinki, Doctoral Programme in Biomedicine
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
University of Helsinki, Klaus Hedman / Principal Investigator
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
University of Helsinki, Klaus Hedman / Principal Investigator
University of Helsinki, Department of Ophthalmology and Otorhinolaryngology
University of Helsinki, Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact











Date: 2019-02
Language: eng
Number of pages: 5
Belongs to series: European Archives of Oto-Rhino-Laryngology
ISSN: 0937-4477
DOI: https://doi.org/10.1007/s00405-018-5250-7
URI: http://hdl.handle.net/10138/300254
Abstract: PurposeApproximately 20% of cancers are estimated to have a viral etiology. We aimed to investigate whether DNA of 8 human parvoviruses [bocavirus 1-4 (HBoV1-4), parvovirus B19 (B19V), protoparvoviruses (bufa-, tusa-, and cutavirus)] and 13 human polyomaviruses (HPyV) can be detected in oropharyngeal and oral cavity squamous cell carcinoma (OPSCC/OSCC), and in juvenile nasopharyngeal angiofibroma (JNA) tissue samples.MethodsFresh samples of seven JNA tissues and ten paired tissues of OSCC/OPSCC tumor and adjacent healthy tissues were collected. DNA extraction and real-time PCRs were performed to detect HBoV1-4, B19V, bufa- tusa- and cutavirus, and HPyV genomes.ResultsJNA specimens were negative for all parvoviruses tested, whereas one JNA sample was Merkel cell polyomavirus (MCPyV) DNA positive. The OSCC/OPSCC samples were negative for the human protoparvoviruses, HBoV1-4, and all human polyomaviruses, except for one patient that was MCPyV DNA positive in both healthy and tumor tissues. Seven OSCC/OPSCC patients were positive for B19V DNA, three of them in both healthy and cancerous tissues and three in only healthy tissues. Three of the B19V DNA-positive patients harbored viral genotype 1, three genotype 2, and one genotype 3B.ConclusionsThese are the first reports of MCPyV and B19V DNA being detected in JNA and OPSCC. The significance of viral DNA positivity is unclear. B19V DNA is known to remain in the tissues lifelong, however, it is of interest that there are some patients with B19 DNA in healthy tissue, but not in the corresponding cancer tissue.
Subject: Juvenile nasopharyngeal angiofibroma
Head and neck cancer
Protoparvovirus
Human parvovirus B19
Bocavirus
Polyomavirus
Merkel cell virus
QUANTITATIVE PCR
PERSISTENCE
INFECTION
HEAD
3125 Otorhinolaryngology, ophthalmology
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